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Start Over Author "Eamonn Maher" Journal journal of nucleic acids
2 results on '"Eamonn Maher"'

1. In Vitro Selection of a Single-Stranded DNA Molecular Recognition Element against Clostridium difficile Toxin B and Sensitive Detection in Human Fecal Matter

Author

Ka Lok Hong, Eamonn Maher, Ryan M. Williams, and Letha J. Sooter

Subjects
Genetics, QH426-470, Biochemistry, QD415-436
Abstract

Toxin B is one of the major virulence factors of Clostridium difficile, a bacterium that is responsible for a significant number of diarrhea cases in acute care settings. Due to the prevalence of C. difficile induced diarrhea, rapid and correct diagnosis is crucial in the disease management. In this study, we have employed a stringent in vitro selection method to identify single-stranded DNA molecular recognition elements (MRE) specific for toxin B. At the end of the 12-round selection, one MRE with high affinity (Kd=47.3 nM) for toxin B was identified. The selected MRE demonstrated low cross binding activities on negative targets: bovine serum albumin, Staphylococcus aureus alpha toxin, Pseudomonas aeruginosa exotoxin A, and cholera toxin of Vibrio cholera. A modified sandwich ELISA assay was developed utilizing the selected ssDNA MRE as the antigen capturing element and achieved a sensitive detection of 50 nM of toxin B in human fecal preparations.

Published
2015
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2. In VitroSelection of a Single-Stranded DNA Molecular Recognition Element againstClostridium difficileToxin B and Sensitive Detection in Human Fecal Matter

Author

Ka Lok Hong, Eamonn Maher, Ryan M. Williams, and Letha J. Sooter

Subjects
lcsh:QH426-470, Article Subject, Virulence, Clostridium difficile toxin B, Biology, medicine.disease_cause, Biochemistry, Microbiology, lcsh:Biochemistry, 03 medical and health sciences, Antigen, medicine, lcsh:QD415-436, Bovine serum albumin, Molecular Biology, Staphylococcus aureus alpha toxin, 030304 developmental biology, 0303 health sciences, 030306 microbiology, Toxin, Cholera toxin, Clostridium difficile, 3. Good health, lcsh:Genetics, biology.protein, Research Article
Abstract

Toxin B is one of the major virulence factors ofClostridium difficile, a bacterium that is responsible for a significant number of diarrhea cases in acute care settings. Due to the prevalence ofC. difficileinduced diarrhea, rapid and correct diagnosis is crucial in the disease management. In this study, we have employed a stringentin vitroselection method to identify single-stranded DNA molecular recognition elements (MRE) specific for toxin B. At the end of the 12-round selection, one MRE with high affinity(Kd=47.3 nM) for toxin B was identified. The selected MRE demonstrated low cross binding activities on negative targets: bovine serum albumin,Staphylococcus aureusalpha toxin,Pseudomonas aeruginosaexotoxin A, and cholera toxin ofVibrio cholera. A modified sandwich ELISA assay was developed utilizing the selected ssDNA MRE as the antigen capturing element and achieved a sensitive detection of 50 nM of toxin B in human fecal preparations.

Published
2015

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