1. PSD-95 Alters Microtubule Dynamics via an Association With EB3.
- Author
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Sweet, Eric S., Previtera, Michelle L., Fernández, Jose R., Charych, Erik I., Chia-Yi Tseng, 1,3, Munjin Kwon, Starovoytov, Valentin, Zheng, James Q., and Firestein, Bonnie L.
- Subjects
MICROTUBULES ,CYTOSKELETON ,ORGANELLES ,DENDRITES ,CYTOPLASM ,NEURONS - Abstract
Little is known about how the neuronal cytoskeleton is regulated when a dendrite decides whether to branch or not. Previously, we reported that postsynaptic density protein 95 (PSD-95) acts as a stop signal for dendrite branching. It is yet to be elucidated how PSD-95 affects the cytoskeleton and how this regulation relates to the dendritic arbor. Here, we show that the SH3 (src homology 3) domain of PSD-95 interacts with a proline-rich region within the microtubule end-binding protein EB3. Overexpression of PSD-95 or mutant EB3 results in a decreased lifetime of EB3 comets in dendrites. In line with these data, transfected rat neurons show that overexpression of PSD-95 results in less organized microtubules at dendritic branch points and decreased dendritogensis. The interaction between PSD-95 and EB3 elucidates a function for a novel region of EB3 and provides a new and important mechanism for the regulation of microtubules in determining dendritic morphology. [ABSTRACT FROM AUTHOR]
- Published
- 2011
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