1. Rationally subdividing the fly nervous system with versatile expression reagents
- Author
-
Julie H. Simpson
- Subjects
0301 basic medicine ,Nervous system ,neural circuit mapping ,animal structures ,Clinical Sciences ,Genetically Modified ,homologous recombination ,Computational biology ,Biology ,Vesicular neurotransmitter transporters ,Animals, Genetically Modified ,Hox genes ,03 medical and health sciences ,Cellular and Molecular Neuroscience ,Genetics ,medicine ,Biological neural network ,Animals ,Drosophila Proteins ,Hox gene ,Transcription factor ,Neurons ,P-element replacement ,Neurology & Neurosurgery ,Neurosciences ,Expression (computer science) ,030104 developmental biology ,medicine.anatomical_structure ,expression patterns ,Drosophila ,Repressor lexA ,Homologous recombination ,Vesicular Neurotransmitter Transport Proteins ,Drosophila Protein ,Transcription Factors - Abstract
The ability to image and manipulate specific cell populations in Drosophila enables the investigation of how neural circuits develop and coordinate appropriate motor behaviors. Gal4 lines give genetic access to many types of neurons, but the expression patterns of these reagents are often complex. Here, we present the generation and expression patterns of LexA lines based on the vesicular neurotransmitter transporters and Hox transcription factors. Intersections between these LexA lines and existing Gal4 collections provide a strategy for rationally subdividing complex expression patterns based on neurotransmitter or segmental identity.
- Published
- 2016
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