1. Physiological requirement for the glutamate transporter dEAAT1 at the adultDrosophila neuromuscular junction
- Author
-
Daniel Cattaert, Magali Iché, Laurent Soustelle, Colette Strambi, Serge Birman, Thomas Rival, Institut de Biologie du Développement de Marseille (IBDM), Aix Marseille Université (AMU)-Centre National de la Recherche Scientifique (CNRS), Institut de génétique et biologie moléculaire et cellulaire (IGBMC), Université Louis Pasteur - Strasbourg I-Institut National de la Santé et de la Recherche Médicale (INSERM)-Centre National de la Recherche Scientifique (CNRS), Centre de Neurosciences Intégratives et Cognitives (CNIC), Centre National de la Recherche Scientifique (CNRS), Laboratoire de neurobiologie des réseaux (LNR), Université Sciences et Technologies - Bordeaux 1 (UB)-Centre National de la Recherche Scientifique (CNRS), Université Sciences et Technologies - Bordeaux 1-Centre National de la Recherche Scientifique (CNRS), and Aix Marseille Université (AMU)-Collège de France (CdF)-Centre National de la Recherche Scientifique (CNRS)
- Subjects
MESH: Rabbits ,Motor nerve ,Stimulation ,Animals, Genetically Modified ,0302 clinical medicine ,MESH: RNA, Small Interfering ,MESH: Gene Expression Regulation, Developmental ,Drosophila Proteins ,Glutamate reuptake ,MESH: Animals ,RNA, Small Interfering ,0303 health sciences ,General Neuroscience ,Age Factors ,Gene Expression Regulation, Developmental ,MESH: Glutamic Acid ,Excitatory Amino Acid Transporter 1 ,Drosophila melanogaster ,medicine.anatomical_structure ,Excitatory postsynaptic potential ,MESH: Neuroglia ,[SDV.NEU]Life Sciences [q-bio]/Neurons and Cognition [q-bio.NC] ,Rabbits ,Neuroglia ,animal structures ,MESH: Drosophila Proteins ,Neuromuscular Junction ,Glutamic Acid ,[SDV.BC]Life Sciences [q-bio]/Cellular Biology ,MESH: Transformation, Genetic ,Biology ,Antibodies ,Neuromuscular junction ,MESH: Drosophila melanogaster ,MESH: Animals, Genetically Modified ,03 medical and health sciences ,Cellular and Molecular Neuroscience ,Transformation, Genetic ,medicine ,Extracellular ,Animals ,MESH: Excitatory Postsynaptic Potentials ,030304 developmental biology ,MESH: Age Factors ,MESH: Antibodies ,fungi ,Excitatory Postsynaptic Potentials ,Transporter ,MESH: Excitatory Amino Acid Transporter 1 ,MESH: Flight, Animal ,Electrophysiology ,nervous system ,Flight, Animal ,MESH: Neuromuscular Junction ,Neuroscience ,030217 neurology & neurosurgery - Abstract
International audience; L-glutamate is the major excitatory neurotransmitter in the mammalian brain. Specific proteins, the Na+/K+-dependent high affinity excitatory amino acid transporters (EAATs), are involved in the extracellular clearance and recycling of this amino acid. Type I synapses of the Drosophila neuromuscular junction (NMJ) similarly use L-glutamate as an excitatory transmitter. However, the localization and function of the only high-affinity glutamate reuptake transporter in Drosophila, dEAAT1, at the NMJ was unknown. Using a specific antibody and transgenic strains, we observed that dEAAT1 is present at the adult, but surprisingly not at embryonic and larval NMJ, suggesting a physiological maturation of the junction during metamorphosis. We found that dEAAT1 is not localized in motor neurons but in glial extensions that closely follow motor axons to the adult NMJ. Inactivation of the dEAAT1 gene by RNA interference generated viable adult flies that were able to walk but were flight-defective. Electrophysiological recordings of the thoracic dorso-lateral NMJ were performed in adult dEAAT1-deficient flies. The lack of dEAAT1 prolonged the duration of the individual responses to motor nerve stimulation and this effect was progressively increased during physiological trains of stimulations. Therefore, glutamate reuptake by glial cells is required to ensure normal activity of the Drosophila NMJ, but only in adult flies.
- Published
- 2006
- Full Text
- View/download PDF