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Your search keyword '"Sharma, Mehar Chand"' showing total 13 results
Start Over Author "Sharma, Mehar Chand" Journal journal of neuro-oncology
13 results on '"Sharma, Mehar Chand"'

2. A prospective phase II trial of response adapted whole brain radiotherapy after high dose methotrexate based chemotherapy in patients with newly diagnosed primary central nervous system lymphoma-analysis of acute toxicity profile and early clinical outcome

Author

Adhikari, Narayan, Biswas, Ahitagni, Gogia, Ajay, Sahoo, Ranjit Kumar, Garg, Ajay, Nehra, Ashima, Sharma, Mehar Chand, Bhasker, Suman, Singh, Manmohan, Sreenivas, Vishnubhatla, Chawla, Rohan, Joshi, Garima, Kumar, Lalit, and Chander, Subhash

Published
2018
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13. <italic>C11orf95-RELA</italic> fusions and upregulated NF-KB signalling characterise a subset of aggressive supratentorial ependymomas that express L1CAM and nestin.

Author

Malgulwar, Prit Benny, Nambirajan, Aruna, Pathak, Pankaj, Faruq, Mohammed, Rajeshwari, Madhu, Singh, Manmohan, Suri, Vaishali, Sarkar, Chitra, and Sharma, Mehar Chand

Abstract

Ependymomas (EPN) show site specific genetic alterations and a recent DNA methylation profiling study identified nine molecular subgroups. C11orf95-RELA and YAP1 fusions characterise the RELA and YAP1 molecular subgroups, respectively, of supratentorial (ST)-EPNs. Current guidelines recommend molecular subgrouping over histological grade for accurate prognostication. Clinicopathological features of ST-EPNs in correlation with C11orf95-RELA and YAP1 fusions have been assessed in only few studies. We aimed to study these fusions in EPNs, and identify diagnostic and prognostic markers. qRT-PCR and Sanger Sequencing for the detection of C11orf95-RELA, YAP1-MAMLD1 and YAP1-FAM118B fusion transcripts, gene expression analysis for NFKB1, and immunohistochemistry for p53, MIB-1, nestin, VEGF, and L1CAM were performed. 88 EPNs (10-Grade I and 78-Grade II/III) from all sites were included. RELA fusions were unique to Grade II/III ST-EPNs, detected in 81.4% (22/27) and 18.5% (5/27) of pediatric and adult ST-EPNs respectively. ST-EPNs harbouring RELA fusions showed frequent grade III histology (81.5%), clear cell morphology (70.3%), upregulated NFKB1 expression, MIB-1 labelling indices (LI) ≥ 10% (77.8%), and immunopositivity for nestin (95.7%), VEGF (72%), L1CAM (79%), and p53 (64%). Presence of RELA fusions, L1CAM immunopositivity and MIB-1 LI ≥ 10% associated with poor outcome. L1CAM showed 81% concordance with RELA fusions. YAP1-MAMLD1 fusion was identified in a single RELA fusion negative adult anaplastic ST-EPN. RELA fusions are frequent in ST-EPNs and associate with poor outcome. L1CAM is a surrogate immunohistochemical marker. RELA fusion positive ST-EPNs strongly express nestin indicating increased stemness. Further evaluation of the interactions between NFKB and stem cell pathways is warranted. [ABSTRACT FROM AUTHOR]

Published
2018
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