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Your search keyword '"Glioblastoma cell"' showing total 4 results
Start Over Descriptor "Glioblastoma cell" Journal journal of neuro-oncology
4 results on '"Glioblastoma cell"'

1. β-Elemene inhibits Hsp90/Raf-1 molecular complex inducing apoptosis of glioblastoma cells.

Author

Zhao, Yong-Shun, Zhu, Ting-Zhun, Chen, Yan-Wei, Yao, Yi-Qun, Wu, Chun-Ming, Wei, Zhen-Qing, Wang, Wei, and Xu, Ying-Hui

Abstract

β-Elemene, an active component of herb medicine Curcuma wenyujin, has been shown to antagonize glioblastoma cells by inducing apoptosis. However, how β-elemene induces apoptosis of these cells remains unclear. In this study, we report that β-elemene disrupted the formation of the Hsp90/Raf-1 complex, a key step in maintaining the conformation stability of Raf-1, and caused deactivation of Raf-1 and inhibition of the ERK pathway, thereby leading to apoptosis of glioblastoma cells. Specifically, treatment of glioblastoma cell lines with β-elemene attenuated phosphorylation of multiple members of the kinase families in the Ras/Raf/MEK/ERK cascade, including Raf-1 and ERK as well as downstream signaling targets such as Bcl-2. These results suggest that the Hsp90/Raf-1 complex could be a promising molecular target for new drug development for the treatment of glioblastoma. [ABSTRACT FROM AUTHOR]

Published
2012
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2. Geranylgeranyltransferase I regulates HIF-1α promoting glioblastoma cell migration and invasion

Author

Yinfu Xu, Yuewen Chao, Qiong Shi, Jiantong Jiao, Zhi Liu, Jianbing Mo, Xiuping Zhou, Wenbin Bian, Rutong Yu, Jinming Qian, Ben Sang, and Xu Song

Subjects
Cancer Research, Blotting, Western, Biology, Transfection, Prenylation, Cell Movement, Cell Line, Tumor, Glioma, medicine, Humans, Transferase, Neoplasm Invasiveness, RNA, Small Interfering, Glioblastoma cell, Geranylgeranyltransferase I, Alkyl and Aryl Transferases, Brain Neoplasms, Cell migration, Hypoxia (medical), Hypoxia-Inducible Factor 1, alpha Subunit, medicine.disease, Cell Hypoxia, Cell biology, Gene Expression Regulation, Neoplastic, Neurology, Oncology, Gene Knockdown Techniques, Protein prenylation, Neurology (clinical), medicine.symptom, Glioblastoma
Abstract

Glioblastoma multiforme is a highly migratory and invasive brain tumor in which hypoxia inducible factor-1α (HIF-1α) plays important roles. However, the underlying mechanisms regulating the action of HIF-1α in glioma cell migration and invasion ability remain unclear. We reported here that HIF-1α was regulated by geranylgeranyltransferase I (GGTI), a protein prenylation transferase, and then promoted glioma cell migration and invasion. The migratory and invasive ability of glioma cells were enhanced by hypoxia treatment but inhibited by down-regulation of HIF-1α. GGTI activity inhibition or GGTI specific β subunit (GGTI β) knocking-down decreased HIF-1α protein level. In addition, down-regulation of GGTI β inhibited migration and invasion of glioma cells under hypoxia, while GGTI β over-expression promoted it. Furthermore, the effect of GGTI β over-expression on cell migration and invasion was abolished by HIF-1α down-regulation. In summary, our study showed, for the first time, that HIF-1α was regulated by protein prenylation transferase GGTI and mediated the effect of GGTI on glioma cell migration and invasion.

Published
2013
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3. MiR-212-3p inhibits glioblastoma cell proliferation by targeting SGK3

Author

Chen Zhong, Weiguang Zhang, Fei Yin, Huaizhang Shi, Chen Shen, Shiguang Zhao, Xiaoxiong Wang, Bingjie Zheng, Kaikai Wang, Xu Hou, Jing Ai, Chenguang Li, Huailei Liu, Jiakang Zhang, Jianing Wu, Xin Chen, and Yaohua Liu

Subjects
Male, Cancer Research, Green Fluorescent Proteins, Mice, Nude, Protein Serine-Threonine Kinases, urologic and male genital diseases, Transfection, Mice, In vivo, Cell Line, Tumor, microRNA, medicine, Animals, Humans, Tumor growth, RNA, Messenger, RNA, Small Interfering, Cell Proliferation, Messenger RNA, Glioblastoma cell, urogenital system, Kinase, Chemistry, Brain Neoplasms, medicine.disease, Xenograft Model Antitumor Assays, nervous system diseases, Gene Expression Regulation, Neoplastic, MicroRNAs, Neurology, Oncology, Cancer research, Linear Models, MiR-212, Female, Neurology (clinical), Glioblastoma
Abstract

Glioblastoma multiforme (GBM) is the most malignant brain tumor in humans. Previous studies have demonstrated that microRNA plays important roles in the development and proliferation of GBM cells. Here we defined the mechanism by which miR-212-3p regulated the proliferation of GBM. In this study, we showed that miR-212-3p expression was significantly down-regulated and negatively correlated with serum and glucocorticoid-inducible kinase 3 (SGK3) in GBM. Either over-expression of miR-212-3p or silence of SGK3 decreased viability of GBM cells. Moreover, miR-212-3p directly bound to 3′UTR of SGK3 and inhibited its mRNA and protein expression. And over-expression of SGK3 rescued the decreased proliferation of GBM cells induced by miR-212-3p. Importantly, miR-212-3p also suppressed tumor growth in vivo. Collectively, our results demonstrated that miR-212-3p inhibited proliferation of GBM cells by directly targeting SGK3, and could potentially serve as a new therapeutic target for GBM.

Published
2014

4. Erratum to: Glioblastoma cell growth is suppressed by disruption of fibroblast growth factor pathway signaling

Author

Sara Grazia Maria Piccirillo, Gregory J. Riggins, Gary L. Gallia, Watcharin Loilome, Colette M. Ap Rhys, Avadhut D. Joshi, and Angelo L. Vescovi

Subjects
Cancer Research, Glioblastoma cell, Fibroblast growth factor receptor 2, Fibroblast growth factor receptor 4, Fibroblast growth factor receptor 3, Biology, Fibroblast growth factor, Pathway signaling, Neurology, Oncology, Cancer research, Growth factor receptor inhibitor, Neurology (clinical), Autocrine signalling
Published
2010
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