1. Antidermatophyte and antimelanogenesis compound from Eleutherine americana grown in Indonesia.
- Author
-
Kusuma IW, Arung ET, Rosamah E, Purwatiningsih S, Kuspradini H, Syafrizal, Astuti J, Kim YU, and Shimizu K
- Subjects
- Animals, Antifungal Agents isolation & purification, Arthrodermataceae physiology, Cell Survival drug effects, Cell Survival physiology, Indonesia, Melanoma, Experimental pathology, Mice, Plant Extracts isolation & purification, Plant Roots, Tumor Cells, Cultured, Antifungal Agents pharmacology, Antineoplastic Agents, Phytogenic isolation & purification, Antineoplastic Agents, Phytogenic therapeutic use, Arthrodermataceae drug effects, Iridaceae, Melanoma, Experimental drug therapy, Plant Extracts pharmacology
- Abstract
An active compound from the bulb of Eleutherine americana L. Merr. (Iridaceae) collected from East Kalimantan, Indonesia, was tested for its antidermatophyte and antimelanogenesis activity. Antifungal assay-directed fractionation of the n-hexane-soluble fraction of the methanolic extract of the bulb of E. americana led to the isolation of 1 as an active compound. The compound was identified as the naphthoquinone eleutherin by EI-MS and (1)H-, (13)C-, and two-dimensional NMR analyses. Antidermatophyte assay of 1 at concentrations of 10, 20, 40, 60, and 80 microg/disk and myconazole, a commercial antidermatophyte, at 10 microg/disk displayed 7, 8, 13, 16, 17, and 14 mm of inhibition zone against Trichophyton mentagrophytes, respectively. In a melanin formation inhibition assay, compound 1 displayed potent antimelanogenesis activity at 5 ppm with low toxicity compared with arbutin, a commercial skin-whitening agent. The results showed the high potential of 1, an active compound from E. americana, to be applied as an antidermatophyte and antimelanogenesis agent.
- Published
- 2010
- Full Text
- View/download PDF