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[Objectives]The paper aimed to identify the grape homeobox(HB)gene family using bioinformatics methods, systematically analyze their sequence and structural characteristics, evolutionary features and expression characteristics, and screen candidate grape HB genes in response to bud dormancy and various stresses. [Methods]Using the local BLAST program, the HB gene in Arabidopsis thaliana was used as the target sequence for comparison with the grape whole genome protein sequence. ExPASy was used to predict the physical and chemical properties of grape HB gene family proteins, and MapGene2Chro was used to locate chromosomes. Based on Neighbour-joining method, the MEGA X software was used to perform phylogenetic tree of these HB genes in grape and A.thaliana. Meanwhile, TBtools software was used to analyze the gene structure, conserved domain and promoter cis-elements characteristics of HB family members. Genomic collinearity of HB gene family members between grape and A.thaliana was analyzed by MCScanX. Based on known transcriptomic data, the expression of HB genes was analyzed under bud dormancy, fruit development and different abiotic stresses. [Results]Eighty HB genes were identified in the grape genome, distributed on 18 chromosomes, which were divided into 9 subfamilies based on evolutionary analysis. The amino acid quantity was 66-1 772, molecular weight was(8.28-198.55)×103 and isoelectric point was 4.60-10.03. The prediction of subcellular localization indicated that the family members were mainly located in chloroplasts. Gene structure analysis revealed that all grape HB members contain exons ranging from 1 to 20 in number. There were 2 pairs of tandem duplicated genes and 23 co-linear pairs in the grape HB gene family, and 82 co-linear pairs between A.thaliana and grape. Promoter cis-elements analysis showed that the HB family contained cis-elements related to light response, plant hormones and stress, and tissue-specific expression. Among grape HB family proteins, KNOX and BEL subfamily members had the closest interaction. Through the analysis of the expression profile of grape HB gene family, it was found that most HB family members were significantly differentially expressed under bud dormancy and drought stress. In addition, seven HB family members were found to be involved in bud dormancy regulation, and VvHB9 and VvHB34 were involved in various abiotic stresses. [Conclusions]In this study, basic information was identified and analyzed on the members of the grape HB family, and several key genes were screened which were involved in the regulation of bud dormancy and response to multiple abiotic stresses based on transcriptomic data. [ABSTRACT FROM AUTHOR]