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Mesenchymal stem cells (MSCs) releases in culture extracellular vesicles called microvesicles (MVs). MVs have beneficial cytokines that prevent progression of the disease and help in the regeneration process. This study is aimed to evaluate the effect of MSCs derived MVs in repair of induced chondral defect in a dog model. Methods: Chondral defects were created surgically ([Formula: see text][Formula: see text]mm) in both femoral condyles of nine dogs, autologous MSCs were isolated and MVs were prepared and injected intraarticularly in the right joint. The left joint was injected with normal saline as control negative. Evaluation of the treatment after first injection was carried out by physical examination and histopathology at different time periods ([Formula: see text]½, 3 and 6 months). Results: Treated joints showed marked degree of cartilage regeneration and restoration of chondral histomorphological picture on the contrary of the control joints that showed deterioration over time and defect filling with only fibrous tissue forming a fibrocartilage at the end of six months period. Conclusion: We demonstrated in this study that administration of MVs was effective on the functional and morphological recovery of the injured cartilage and could be exploited as a cell free therapeutic approach in regenerative medicine.