1. Investigation on the spectral properties of 2D asynchronous fluorescence spectra generated by using variable excitation wavelengths as a perturbation
- Author
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Jinguang Wu, Juan Feng, Anqi He, Ran Guo, Yizhuang Xu, Yongju Wei, Jing-Dan Wang, and Isao Noda
- Subjects
Anthracene ,Cyclohexane ,010405 organic chemistry ,Organic Chemistry ,Analytical chemistry ,010402 general chemistry ,01 natural sciences ,Fluorescence ,Molecular physics ,Spectral line ,0104 chemical sciences ,Analytical Chemistry ,Inorganic Chemistry ,chemistry.chemical_compound ,Wavelength ,chemistry ,Molecule ,Emission spectrum ,Spectroscopy ,Excitation - Abstract
Properties of 2D asynchronous spectra generated from a series of fluorescence emission spectra are investigated. Variable excitation wavelengths are utilized as an external perturbation. Based on the results of mathematical analysis and computer simulation, we find that no cross peak will be produced on the 2D asynchronous spectrum if the fluorescent solute under investigation occurs in a single micro-environment. The observation of cross peaks implies that the fluorescent molecule may occur in different micro-environments in a solution. Based on these results, we use 2D asynchronous spectra to investigate the emission spectra of anthracene dissolved in cyclohexane. When the concentration of anthracene is low, no cross peak is produced in the resultant 2D asynchronous spectrum, confirming that anthracene is dissolved as single molecule in the solution. As the concentration elevated, cross peaks appear in the corresponding 2D asynchronous spectra. A plausible explanation of this phenomenon is that anthracene may undergo aggregation via π-π interaction or π-C-H interaction.
- Published
- 2016