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Your search keyword '"Pasquali, D"' showing total 5 results
Start Over Author "Pasquali, D" Journal journal of molecular endocrinology
5 results on '"Pasquali, D"'

4. Sodium butyrate/retinoic acid costimulation induces apoptosis-independent growth arrest and cell differentiation in normal and ras-transformed seminal vesicle epithelial cells unresponsive to retinoic acid

Author

Daniela Pasquali, Francesco Morelli, Antonio Agostino Sinisi, Antonio Bellastella, S Metafora, Elisabetta Buommino, Buommino, Elisabetta, Pasquali, D, Sinisi, A. A, Bellastella, A, Morelli, F, Metafora, S., Pasquali, Daniela, and Sinisi, Aa

Subjects
Male, DNA Primer, Receptors, Retinoic Acid, Cellular differentiation, Retinoic acid, Down-Regulation, Apoptosis, Tretinoin, Biology, chemistry.chemical_compound, Endocrinology, Downregulation and upregulation, Seminal Vesicle, Animals, RNA, Messenger, Receptor, Molecular Biology, Cell Line, Transformed, DNA Primers, Epithelial Cell, Transglutaminases, Base Sequence, Cell growth, Animal, Apoptosi, Seminal Vesicles, Sodium butyrate, Butyrate, Cell Differentiation, Epithelial Cells, Genes, ra, Transglutaminase, Cell biology, Rats, Butyrates, Genes, ras, Biochemistry, chemistry, Rat, Signal transduction, Cell Division
Abstract

Retinoic acid (RA) and sodium butyrate (NaB) are regulators of cell growth and differentiation. We studied their effect on normal (SVC1) or v-Ki-ras-transformed (Ki-SVC1) rat seminal vesicle (SV) epithelial cell lines. The treatment of these cells with 10(-((7( M RA did not produce significant changes in the morphological and biochemical parameters analyzed. When RA was used in combination with 2 mM NaB, the treatment induced substantial morphological changes, apoptosis-independent growth arrest, up-regulation of tissue transglutaminase (tTGase), and down-regulation of beta and gamma RA receptor (RAR) mRNA expression. The same cells did not express RAR alpha either before or after NaB/RA treatment. A similar treatment did not change the amount of mRNA coding for the protein SV-IV (a typical differentiation marker of the SV epithelium) in normal or ras-transformed cells nor the level of v-Ki-ras mRNA in Ki-SVC1 cells. These findings suggest that a defective RA/RARs signaling pathway is probably the biochemical condition that underlies the unresponsiveness to RA of our in vitro culture system, and indirectly points to the possibility that the NaB/RA-induced effects were brought about by a cooperation at the transcription level between the histone deacetylase inhibitory activity of NaB and the ability of RA/RAR to modulate the expression of various genes involved in the control of cell growth and differentiation.

Published
2000

5. Effects of somatostatin analog SOM230 on cell proliferation, apoptosis, and catecholamine levels in cultured pheochromocytoma cells.

Author

Pasquali D, Rossi V, Conzo G, Pannone G, Bufo P, De Bellis A, Renzullo A, Bellastella G, Colao A, Vallone G, Bellastella A, and Sinisi AA

Subjects
Adult, Caspase 3 metabolism, Cell Proliferation drug effects, Cell Survival drug effects, Gene Expression Regulation, Neoplastic drug effects, Humans, Immunohistochemistry, Intracellular Space drug effects, Intracellular Space metabolism, Middle Aged, Octreotide pharmacology, Pheochromocytoma enzymology, Pheochromocytoma genetics, Poly(ADP-ribose) Polymerases metabolism, Receptors, Somatostatin genetics, Receptors, Somatostatin metabolism, Somatostatin genetics, Somatostatin pharmacology, Tumor Cells, Cultured, Apoptosis drug effects, Catecholamines metabolism, Pheochromocytoma pathology, Somatostatin analogs & derivatives
Abstract

Surgery is the primary therapy for pheochromocytoma (PHEO), a catecholamine-producing tumor. Benign and malignant PHEO could develop recurrences, and the intraoperative risk of recurrent PHEO is an important unresolved issue. Non-surgical treatments of PHEO recurrence would therefore better prepare patients for reintervention as well as provide them with palliative management. We investigated the effects of the new somatostatin analog (pasireotide) SOM230 versus octreotide (OCT) in primary PHEO cell cultures (Pheo-c). Pheo-c from six benign surgical samples were set up and characterized by immunocytochemistry. Real-time PCR, using both PHEO tissues and Pheo-c, showed different levels of somatostatin receptor(1-5) mRNA expression. Cells treated with various doses of OCT or SOM230 for 48 and 72 h were analyzed to assess their effects on cell proliferation and apoptosis and catecholamine levels. Even if reduction of cell viability was observed in Pheo-c treated for 48 h with either OCT or SOM230 and this effect increased after 72 h, a more significant inhibition of cell growth as well as a significantly higher induction of apoptosis was seen in Pheo-c treated with SOM230 versus OCT. In particular, apoptosis in Pheo-c was detected after 48 h and was associated with increased expression and activation of caspase-3 and cleaved poly(ADP-ribose) polymerase. OCT 10(-6) M and SOM230 10(-7) M significantly reduced catecholamine levels. Our results indicate that while both OCT and SOM230 modulate cell growth and apoptosis and catecholamine levels in Pheo-c through specific receptors, SOM230 is more effective. This improves our knowledge on the mechanism of SOM230 action in PHEO and supports a possible therapeutic use in benign PHEO recurrence.

Published
2008
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