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Objective To establish the automatic synthesis method and quality control method of 18FDPA- 714. Methods Using Sumitomo CFN-F100 multifunctional synthesis module in Japan, the automatic synthesis program was edited independently. 18F-produced by cyclotron HM-10 was captured by QMA column, then eluted, dried and dewatered, and nucleophilic substitution reaction was carried out with the precursor. The crude product was transferred to semi-preparative high-performance liquid chromatography(HPLC) for separation and purification, the peak of the product was collected, diluted with water, transferred to the C18 column, then washed with water, and finally eluted with 2 mL ethanol, diluted with normal saline, and obtained the product through the sterile filter membrane. The pH value, radiochemical purity, stability and bacterial endotoxin were measured. Results Online automatic preparation of 18F-DPA-714 radiochemical purity was >99%,and synthesis efficiency without attenuation correction was 18%-36%(n=20), the specific activity could reach 675 GBq/mg, and the entire preparation time was about 55 min. Conclusion The online automatic preparation method of 18F-DPA-714 is simple, easy to operate, safe, favorable for radiation protection of workers, stable in synthesis efficiency, high in radiochemical purity, high in specific activity, and qualified in various indexes, which can meet the needs of clinical use [ABSTRACT FROM AUTHOR]