Search

Objective To screen for differentially expressed genes( DEGs) involved in the development of spinal cord injury using bioinformatics methods, and to explore the changes of lipopolysaccharide( LPS) activated BV2 cells DEGs in vitro, in order to provide a new target for the treatment of spinal cord injury. Methods The gene-chip data were downloaded from GEO database, and the samples in the data set were divided into the spinal cord injury Day 2 group and the Day 5 group. R software was used to process the batch effect between the samples from different data sets, and the expression data of the gene-chip was standardized. R software was used to analyze the standardized gene expression matrix to obtain the differential genes. The differential genes were imported into the DAVID database for gene ontology( GO) analysis, and the pathway analysis was performed through the KEGG database. The protein-protein interactions( PPI) network was constructed using STRING protein database, and 10 Hub genes were analyzed by Cystoscope software. Real-time fluorescence quantitative polymerase chain reaction( RT-qPCR) was used to analyze the top two genes with high scores in the Hub gene of BV2 microglia activated by LPS. Results Compared with the Day 2 group, there were 340 DEGs in the Day 5 group. Analysis of the bioinformation showed that DEGs were enriched in cell inflammatory response and substance metabolism. A total of 10 central nodes were obtained through module analysis with STRING software. KEGG re-analysis of 10 genes revealed that CXCL10,CCR3,CCR10 and CCL2 genes were significantly enriched in chemokine signaling pathway and cytokine-cytokine receptor interaction. In vitro experiment, compared with normal group, the expression levels of inflammatory cytokines [interleukin-1β( IL-1β),IL-6 and tumor necrosis factor α( TNF-α) ] and mRNA expressions of chemokines CXCL10,CCR9/10 and CCL2 were increased in the LPS group, with statistical significance( P＜0.05) .Conclusion CXCL10,CCR3,CCR10 and CCL2 chemokine signaling pathways and cytokine-cytokine receptor interactions may be closely related to the development mechanism of spinal cord injury.CXCL10,CCR9/10 and CCL2 mediate the inflammatory responses of BV2 cells. [ABSTRACT FROM AUTHOR]

Objective To increase the comfort level of the patients with breast cancer by improving the postoperative body posture for increasing the nursing satisfactory degree. Methods One hundred and four cases of breast cancer treated in this hospital from October 2015 to May 2017 were divided into the control group（51 cases）and observation group（53 cases）.The control group adopted the recumbent position without pillow after operation，while the observation group adopted the horizontal position by rising the bed head at 20°-30°. The postoperative headache，dizzness，nausea，vomiting，pain of neck，back and shoulder and comfort were compared between the two groups. Results The postoperative headache，dizzness，nausea，vomiting， pain of neck，back and shoulder and comfort had statistical difference between the observation group and control group（P<0.05）； the incidence rate of involved limb swelling in the observation group was significantly lower than that in the control group，the difference was statistically significant（P<0.05）. Conclusion The position improvement after breast cancer operation can allevi-ate the patient′s pain，enhances the comfort and increases the nursing satisfaction [ABSTRACT FROM AUTHOR]