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Start Over Author "Tiziana M. Cafarelli" Journal journal of microbiological methods
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1. SOE-LRed: A simple and time-efficient method to localize genes with point mutations onto the Escherichia coli chromosome

Author

Veronica G. Godoy, Tiziana M. Cafarelli, and Ryan W. Benson

Subjects
Microbiology (medical), Genetics, Microbial, medicine.disease_cause, Microbiology, Polymerase Chain Reaction, Article, law.invention, Chromosome (genetic algorithm), law, medicine, Escherichia coli, Point Mutation, Gene, Molecular Biology, Polymerase chain reaction, Genetics, Recombination, Genetic, biology, Point mutation, Escherichia coli Proteins, Chromosome Mapping, Chromosomes, Bacterial, biology.organism_classification, Enterobacteriaceae, Artificial Gene Fusion, Transformation (genetics), Genes, Bacterial, Mutant Proteins, Homologous recombination
Abstract

We report a powerful method to replace wild type genes on the chromosome of Escherichia coli. Employing a unique form of PCR, we generate easily constructible gene fusions bearing single point mutations. Used in conjunction with homologous recombination, this method eliminates cloning procedures previously used for this purpose.

Published
2010

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