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Start Over Author "Shin Sakuma" Journal journal of microbiological methods
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1. A new culturing method for production of filamentous hemagglutinin of Bordetella pertussis

Author

Yuji Sato, Shin Sakuma, Atsushi Imaizumi, Akihiro Ginnaga, and Yoji Suzuki

Subjects
Microbiology (medical), Bordetella pertussis, Filamentous haemagglutinin adhesin, Biology, biology.organism_classification, Molecular Biology, Microbiology
Abstract

The addition of cyclodextrins, especially heptakis (2,6- O -dimethyl)β-cyclodextrin (MeβCD), to synthetic medium, such as Stainer-Scholte medium (D.W. Stainer and M.J. Scholte, J. Gen. Microbiol. 63: 211–220) greatly stimulated the production of filamentous hemagglutinin (F-HA) by Bordetella pertussis Tohama phase I under shaking conditions. Purified F-HA from this culturing system was demonstrated immunochemically and morphologically to be identical with that from static culture.

Published
1984
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