1. Development of a novel DNA microarray to detect bacterial pathogens in patients with chronic obstructive pulmonary disease (COPD).
- Author
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Curran T, Coulter WA, Fairley DJ, McManus T, Kidney J, Larkin M, Moore JE, and Coyle PV
- Subjects
- Chlamydophila pneumoniae genetics, Chlamydophila pneumoniae isolation & purification, Coxiella burnetii genetics, Coxiella burnetii isolation & purification, DNA, Bacterial analysis, DNA, Bacterial genetics, Gram-Negative Bacteria genetics, Gram-Negative Bacterial Infections etiology, Gram-Positive Bacterial Infections etiology, Haemophilus genetics, Haemophilus isolation & purification, Humans, Legionella pneumophila genetics, Legionella pneumophila isolation & purification, Moraxella catarrhalis genetics, Moraxella catarrhalis isolation & purification, Mycoplasma pneumoniae genetics, Mycoplasma pneumoniae isolation & purification, Pseudomonas aeruginosa genetics, Pseudomonas aeruginosa isolation & purification, Pulmonary Disease, Chronic Obstructive complications, Respiratory Tract Infections etiology, Sensitivity and Specificity, Species Specificity, Sputum microbiology, Streptococcus pneumoniae genetics, Streptococcus pyogenes genetics, Gram-Negative Bacteria isolation & purification, Gram-Negative Bacterial Infections diagnosis, Gram-Positive Bacterial Infections diagnosis, Oligonucleotide Array Sequence Analysis methods, Pulmonary Disease, Chronic Obstructive microbiology, Respiratory Tract Infections diagnosis, Streptococcus pneumoniae isolation & purification, Streptococcus pyogenes isolation & purification
- Abstract
A novel microarray was constructed with DNA PCR product probes targeting species specific functional genes of nine clinically significant respiratory pathogens, including the Gram-positive organisms (Streptococcus pneumoniae, Streptococcus pyogenes), the Gram-negative organisms (Chlamydia pneumoniae, Coxiella burnetii Haemophilus spp., Legionella pneumophila, Moraxella catarrhalis, and Pseudomonas aeruginosa), as well as the atypical bacterium, Mycoplasma pneumoniae. In a "proof-of-concept" evaluation of the developed microarray, the microarray was compared with real-time PCR from 14 sputum specimens from COPD patients. All of the samples positive for bacterial species in real-time PCR were also positive for the same bacterial species using the microarray. This study shows that a microarray using PCR probes is a potentially useful method to monitor the populations of bacteria in respiratory specimens and can be tailored to specific clinical needs such as respiratory infections of particular patient populations, including patients with cystic fibrosis and bronchiectasis., (Copyright 2010 Elsevier B.V. All rights reserved.)
- Published
- 2010
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