1. The anti-inflammatory effect of kaempferol in aged kidney tissues: the involvement of nuclear factor-kappaB via nuclear factor-inducing kinase/IkappaB kinase and mitogen-activated protein kinase pathways
- Author
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Byung Pal Yu, Min-Sun Kim, Hae Young Chung, Bokyoung Sung, Nam Deuk Kim, Mi Kyung Kim, Jae Sue Choi, Eun Kyeong Lee, Hyoung-Sam Heo, Stephen D. Anton, Jaewon Lee, and Min Ju Park
- Subjects
Male ,Aging ,T-Lymphocytes ,Anti-Inflammatory Agents ,Medicine (miscellaneous) ,Phytoestrogens ,IκB kinase ,Biology ,Kidney ,Antioxidants ,Cell Line ,Rats, Sprague-Dawley ,chemistry.chemical_compound ,tert-Butylhydroperoxide ,Gene expression ,Animals ,Kaempferols ,Protein kinase A ,Transcription factor ,Chemokine CCL2 ,Nutrition and Dietetics ,Kinase ,Plant Extracts ,NF-kappa B ,Molecular biology ,Rats ,Nitric oxide synthase ,chemistry ,Mitogen-activated protein kinase ,Models, Animal ,biology.protein ,Mitogen-Activated Protein Kinases ,Kaempferol ,Full Communications - Abstract
Kaempferol, one of the phytoestrogens, is found in berries and Brassica and Allium species and is known to have antioxidative and anti-inflammatory properties. In the present study, we examined the molecular mechanisms underlying the anti-inflammation effect of kaempferol in an aged animal model. To examine the effect of kaempferol in aged Sprague-Dawley rats, kaempferol was fed at 2 or 4 mg/kg/day for 10 days. The data show that kaempferol exhibited the ability to maintain redox balance. Kaempferol suppressed nuclear factor-kappaB (NF-kappaB) activation and expression of its target genes cyclooxygenase-2, inducible nitric oxide synthase, monocyte chemoattractant protein-1, and regulated upon activation, and normal T-cell expressed and secreted in aged rat kidney and in tert-butylhydroperoxide-induced YPEN-1 cells. Furthermore, kaempferol suppressed the increase of the pro-inflammatory NF-kappaB cascade through modulation of nuclear factor-inducing kinase (NIK)/IkappaB kinase (IKK) and mitogen-activated protein kinases (MAPKs) in aged rat kidney. Based on these results, we concluded that anti-oxidative kaempferol suppressed the activation of inflammatory NF-kappaB transcription factor through NIK/IKK and MAPKs in aged rat kidney.
- Published
- 2009