1. Synthesis of Natural Product-Inspired Inhibitors of Mycobacterium tuberculosis Mycothiol-Associated Enzymes: The First Inhibitors of GlcNAc-Ins Deacetylase
- Author
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Brandon J. Fetterolf, Maria-Teresa Gutierrez-Lugo, Syed Shazad-ul-Hussan, Jeremy A. Smith, Matthew Moravec, Carole A. Bewley, Belhu B. Metaferia, and Satyajit Ray
- Subjects
Models, Molecular ,Stereochemistry ,Amidohydrolases ,Chemical library ,Amidase ,chemistry.chemical_compound ,Bacterial Proteins ,Oxazines ,Drug Discovery ,Cysteine ,Furans ,Oxazoles ,Pyrans ,Antibacterial agent ,chemistry.chemical_classification ,Sulfonamides ,Binding Sites ,Natural product ,biology ,Glycopeptides ,Active site ,Stereoisomerism ,Isoxazoles ,Mycobacterium tuberculosis ,Mycothiol ,Enzyme ,chemistry ,Biochemistry ,Thioglycosides ,Enzyme inhibitor ,biology.protein ,Molecular Medicine ,Inositol ,Protein Binding - Abstract
Synthesis and evaluation of a chemical library of inhibitors of the mycothiol biosynthesis enzyme GlcNAc-Ins deacetylase (MshB) and the mycothiol-dependent detoxification enzyme mycothiol- S-conjugate amidase (MCA) from Mycobacterium tuberculosis are reported. The library was biased to include structural features of a group of natural products previously shown to competitively inhibit MCA. Molecular docking studies that reproducibly placed the inhibitors in the active site of the enzyme MshB reveal the mode of binding and are consistent with observed biological activity.
- Published
- 2007
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