1. Monodisperse collagen-gelatin beads as potential platforms for 3D cell culturing
- Author
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Fiona M. Watt, Shaohua Ma, Andreas Fery, Wilhelm T. S. Huck, Martin Peter Neubauer, Manuela Natoli, and Xin Liu
- Subjects
Materials science ,food.ingredient ,Microfluidics ,Dispersity ,technology, industry, and agriculture ,Biomedical Engineering ,Nanotechnology ,General Chemistry ,General Medicine ,Gelatin ,Buffer (optical fiber) ,medicine.anatomical_structure ,food ,Chemical engineering ,Cell culture ,medicine ,General Materials Science ,Fibroblast ,Porosity - Abstract
A droplet-based microfluidics technique is used to produce monodisperse, 80 μm collagen–gelatin beads with tunable mechanical properties in the range of 1–10 kPa after photo-crosslinking. The gel beads are porous, mechanically robust and stable in buffer, but can be degraded enzymatically. Encapsulated fibroblast cells maintain 70% viability after one-week encapsulation and preliminary results show that the degree of spreading of cells in gels is correlated with the stiffness of the material.
- Published
- 2020