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The ingenious design of multi-functional materials to simultaneously achieve the accurate detection of targets and effective treatment of target-related diseases is of great significance for both practical and clinical applications. Accordingly, based on their advantages of facile synthesis and function designability, functional nanomaterials have become promising candidates for integrating multi-functionality into one platform, especially carbon dot (CD)-based materials. Herein, deferoxamine (DFO)-inspired CDs with integrated "sense and treatment" potential were elaborately designed and fabricated via a one-pot hydrothermal synthesis by employing l-aspartic acid (Asp) and 2,5-diaminobenzenesulfonic acid (DABSA) as the reactants. A series of characterization results distinctly confirmed that the synthesized CDs possessed a unique chemical composition, uniform spherical morphology (diameter of around 5 nm) and good dispersibility in aqueous solution, exhibiting excellent fluorescence stability under different conditions. Owing to the complexation interaction between Fe3+ and the functional groups of CDs, the selective and sensitive detection of Fe3+ could be successfully realized through fluorescent and colorimetric dual-mode detection based on the statistic quenching in the initial stage, and subsequently the FRET process. Furthermore, these CDs could be utilized for cellular imaging and effective Fe3+ detection due to their outstanding biocompatibility and cytoplasmatic distribution. More significantly, these DFO-inspired CDs could remarkably promote the proliferation of various mammalian cells. Particularly, the results in this work obviously indicated that this type of CDs could weaken the damage of Fe3+ towards the physiological behaviors of cells, helping the cells to regain their capability of differentiation after ferric toxicosis. Therefore, this work presents an original approach for the design and fabrication of multi-functional materials according to the "one stone, three birds" strategy, which may be an optional solution to develop various multi-functional platforms for disease diagnosis and corresponding clinical treatment.

Although carbon dots (CDs) have been synthesized and applied in a variety of biological fields, such as disease diagnosis and gene/drug delivery, the exploration of facile bioinspired synthesis and applications of CDs is still of great significance. Particularly, recent increasing research has clearly confirmed that nanomaterials can affect a series of physiological behaviors and functions of mesenchymal stem cells (MSCs) (e.g., differentiation and pluripotency). Therefore, it is very important to develop multifunctional nanomaterials to simultaneously realize the cellular labelling and regulation of MSC behaviors in practical applications. Herein, sulfonated glycosaminoglycan-bioinspired CDs as bi-functional nanomaterials were ingeniously designed for cellular imaging and promoting the differentiation of rat bone MSCs (rBMSCs) in different culture media, which simultaneously met the two fundamental requirements in the field of MSC-based treatments (e.g., precisely directing the differentiation of MSCs and effective cellular labeling). These bifunctional CDs were successfully prepared via one-pot hydrothermal synthesis by using d-glucosamine hydrochloride (GA·HCl) and sodium p-styrenesulfonate (NaSS) as the reactants. The synthesized CDs with a uniform particle size (around 4 nm) dispersed well in aqueous solutions and exhibited remarkable fluorescence stability under different conditions. Additionally, cell viability and proliferation results demonstrated that the CDs possessed good biocompatibility, having negligible effects on the self-renewal potential of rBMSCs. The as-prepared CDs presented a cytoplasmatic distribution after being ingested by rBMSCs; thus, they are particularly suitable for cellular imaging. More importantly, the addition of CDs to osteogenic and chondrogenic induction media (OIM and CIM), respectively, was capable of effectively promoting the osteogenic and chondrogenic differentiation of rBMSCs due to the generation of reactive oxygen species (ROS) while having no influence on their pluripotency. In brief, this study not only implements a cellular labeling method based on CDs that were synthesized by a biomimicking strategy, but also paves a new way to regulate the differentiation of MSCs by designing multifunctional nanomaterials; this will enable the extensive development of facile synthesis methods and new applications of CDs and will also provide some research foundations for MSC-based fields.