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The development of a responsive fluorescent probe for the detection of a particular biomolecule in a specific site at the desired moment is important in the fields of bioanalysis and imaging, molecular biology and biomedical research. In this work, we report the development of a remote-light activatable nanoprobe for the fluorescence detection of sulphite in pure aqueous solution and its imaging applications in living cells. The nanoprobe, Poly-Cm-SP, is fabricated simply by wrapping photochromic molecules (Cm-SP) into a polymer nanoparticle. Upon alternate UV/Vis light irradiation for several seconds, the Poly-Cm-SP nanoprobe exhibits red/blue fluorescence switch due to the inactive/active FRET processes from coumarins to the SP/MR isomers of the photochromic molecule. In the presence of sulphite, the specific reaction of sulphite with the electron deficit "CC" bond of the MR isomer occurs, resulting in an inefficient FRET process and thus exhibiting a constant "ON" blue channel fluorescence signal. After UV-light irradiation, the formation of activated Poly-Cm-MR

An artificial protein-probe hybrid, Cm-Np-B@BSA, was prepared via host-guest interactions between hydrogen peroxide (H2O2)-responsive Cm-Np-B molecule and bovine serum albumin (BSA). The Cm-Np-B@BSA probe exhibited high sensitivity and selectivity towards H2O2 under physiological conditions and had excellent biocompatibility, allowing for sensitive ratiometric detection and imaging of endogenous H2O2 in live cells.