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Abstract: Long-term variability of the biogeochemical properties during the formation of central waters in the Eastern North Atlantic were analyzed between 42–47°N and 10–20°W from the dataset gathered during the Galicia VII (GVII) and C. Darwin 58/59 (CD58/59) cruises. These cruises that showed important changes in the thermohaline properties and the nutrient abundance of the upper layers were carried out under contrasting conditions of the North Atlantic Oscillation (NAO) index. The different climate forcing led a meridional shift of the transition zone between the formation regions of subpolar and subtropical Eastern North Atlantic Central Water (ENACWp and ENACWt, respectively). This displacement conditioned the presence of each ENACW in the study region and so the thermohaline and biogeochemical properties. The effect of the observed variability at decadal scale on the air–sea CO2 gradient (ΔfCO2) and exchange (F CO2) was analyzed using 1D model approach throughout 11 weekly-steps that simulated the development of a spring bloom during the shoaling of the mixed layer. The outputs of the model showed an intensification of the ocean CO2 uptake due to higher biological CO2 drawdown, during positive NAO conditions and its weakening under negative NAO influence. [ABSTRACT FROM AUTHOR]