1. Preparation and properties of bio-compatible magnetic Fe3O4 nanoparticles
- Author
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Chan, H.T., Do, Y.Y., Huang, P.L., Chien, P.L., Chan, T.S., Liu, R.S., Huang, C.Y., Yang, S.Y., and Horng, H.E.
- Subjects
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NANOPARTICLES , *ALKALINE phosphatase , *VITAMIN B complex , *ENZYME-linked immunosorbent assay - Abstract
Abstract: In this work, we study the preparation and properties of bio-compatible magnetic nanoparticles for immunoassay and DNA detection. The magnetite (Fe3O4) nanoparticles were prepared by a chemical co-precipitation method and dextran was selected as the surfactant to suspend the nanoparticles. Suspended particles associated with avidin followed by biotin were qualitatively analyzed by enzyme-linked immunosorbent assay (ELISA) method. We found further the ethylenediamine blocked activated residual groups efficiently, hence enhancing the attachment of biotin for probing the avidin. [Copyright &y& Elsevier]
- Published
- 2006
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