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The separation and determination of various monosaccharides and oligosaccharides by different HPLC techniques, published in the period from the second part of 1983 up to December 1987, are reviewed. However, several earlier works of particular significance are also covered. This review includes a systematic description of the important elements in an advanced HPLC system for analysis of saccharides: stationary phases for columns, specific mobile phases, the use of precolumn or postcolumn derivatization and the choise of detector for each specific analysis. the most significant developments of the last decade are the increasing use of bonded-phase

The present work demonstrates a method by which natural, non-UV absorbing penicillins can be derivatized to form phenacyl esters. The reaction is rapid and essentially completed in twenty minutes. A straight line correlation was found between the peak height of a phenacyl ester derivative and the amount of the penicillin, a fact which attests to the applicability of the method for quantisation. The phenacyl esters of the penicillins studied here were separated on a reversed phase column.