1. Native LDL potentiate TNF alpha and IL-8 production by human mononuclear cells
- Author
-
Mihai G. Netea, Jos W. M. van der Meer, Anton F. H. Stalenhoef, Liesbeth E. H. Jacobs, Peter H.G.M. Willems, Anneke Hijmans, Pierre N.M. Demacker, Trees J.G. Verver-Jansen, Lambertus H.J. van Tits, Joost G. J. Hoenderop, and Bart Jan Kullberg
- Subjects
Lipopolysaccharides ,Cell Membrane Permeability ,Patch-Clamp Techniques ,Lipopolysaccharide ,Physiology ,medicine.medical_treatment ,Regulation of salt and water reabsorption in the renal collecting duct ,Anti-Inflammatory Agents ,Myocardial Ischemia ,Lipopolysaccharide Receptors ,Ca2+ mobilization ,Kidney ,Physiology, General (Non MeSH) ,Biochemistry ,General (Non MeSH) ,Kidney Concentrating Ability ,Signaaltransductie en ionentransport ,chemistry.chemical_compound ,Endocrinology ,Pancreatic Juice ,Adrenal Cortex Hormones ,Gastric ,Chronic ,Urinary Tract ,Cells, Cultured ,Peripheral Vascular Diseases ,Lipoproteins and atherosclerose ,Phagocytes ,Fatigue Syndrome, Chronic ,Cultured ,Gastric Juice ,Hypothalamic Hormones ,The role of cytokines in the pathophysiology of febrile illnesses and in host defense against infections ,Stomach ,Signal Transduction and Ion Transport ,Free Radical Scavengers ,Geneeskunde: algemeen ,Water-Electrolyte Balance ,Fatigue Syndrome ,Life sciences ,Financial Economics ,Periodic Disease ,Lipoproteins, LDL ,Cytokine ,Hyperlipidemia ,Regulatie water en zouttransport in de verzamelbuis van de nier ,Antirheumatic Agents ,Antilipemic Agents ,Tumor necrosis factor alpha ,Kidney Diseases ,Cholecystokinin ,Infection ,Baculoviridae ,Signal Transduction ,medicine.medical_specialty ,Thapsigargin ,CD14 ,Cells ,Urogenital System ,QD415-436 ,Lipoproteïnen en atherosclerose ,Biology ,Opportunistic Infections ,tumor necrosis factor α ,Peripheral blood mononuclear cell ,Cell Physiology (Non MeSH) ,Proinflammatory cytokine ,Parietal Cells, Gastric ,De rol van cytokinen in de pathofysiologie van koortsende ziekten en in de afweer tegen infecties ,Internal medicine ,medicine ,Humans ,Interleukin 8 ,Bescherming en bevordering van de menselijke gezondheid ,Calcium Signaling ,Hemic and Immune Systems (Non MeSH) ,Ion Transport ,Tumor Necrosis Factor-alpha ,Parietal Cells ,Interleukin-8 ,Immunologic Deficiency Syndromes ,Cell Biology ,Hormones ,Oxidative Stress ,chemistry ,Anti-Inflammatory Agents, Antirheumatic Agents, and Inflammation Mediators (Non MeSH) ,and Inflammation Mediators (Non MeSH) ,Leukocytes, Mononuclear ,Calcium ,low density lipoprotein - Abstract
Contains fulltext : 185418.pdf (Publisher’s version ) (Open Access) Native LDL (nLDL) increases expression of adhesion molecules on endothelial cells through induction of Ca(2+) mobilization. Ca(2+) mobilization is also involved in the induction of proinflammatory cytokines, important mediators involved in atherogenesis. The aim of the study was to evaluate the capacity of nLDL to affect spontaneous and lipopolysaccharide (LPS)-stimulated cytokine production. Preincubation of human peripheral blood mononuclear cells (PBMC) with nLDL for 24 h did not influence spontaneous production of tumor necrosis factor alpha (TNF alpha) or interleukin-8 (IL-8), but significantly potentiated LPS-induced production of these cytokines. nLDL preincubation of PBMC did not increase the expression of the LPS receptors Toll-like receptor-4, CD14, or CD11c/CD18. Potentiation of cytokine production by nLDL was mediated through induction of Ca(2+) mobilization, because: a) nLDL induced a sustained pattern of repetitive Ca(2+) transients in human PBMC; b) the Ca(2+) chelator fura 2-acetoxymethyl ester, 1,2-bis(2-aminophenoxy)ethane-N,N,N',N'-tetraacetic acid, an intracellular Ca(2+) chelator, inhibited the potentiating effect of nLDL on LPS-induced cytokine synthesis; c) induction of Ca(2+) mobilization by thapsigargin potentiated LPS-induced cytokine production. nLDL are able to potentiate LPS-induced production of cytokines by human PBMC, and this effect is probably mediated through induction of Ca(2+) mobilization. This may represent an important pathogenetic mechanism in atherogenesis induced by hyperlipoproteinemia.
- Published
- 2002
- Full Text
- View/download PDF