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Objective To study the effect of methamphetamine on the expression of DA，5-HTand TNF-α in rats hippocampus. Methods (1) The in vivo toxicity model was established by injecting MA 15 mg/(kg.time)， two times a day for 5 days. The in vitro toxicity model was established by incubating MA with mixed hippocampal neurons/microglia for 24 hours. (2) ELISA was used to detect the content of DA， 5-HT and TNF-α in rat hippocampus and cell culture supernatant. Results On the fifth day，Methamphetamine treated rats showed hair errecting， weight loss， rapid head shaking，circling or head dorsal movement， with a stereotyped score of 5. The levels of DA and 5-HT decreased， and the content of TNF-alpha elevated in rats hippocampus of MA group . The levels of DA ， 5-HT decreased and the content of TNF- alpha increased in cell cultural supernatants of MA group 24 hours. Conclusion The changes of DA， 5-HT and TNF- alpha are involved in rat hippocampal damage induced by methamphetamine. [ABSTRACT FROM AUTHOR]