Search

Objective　To observe whether Huashi Runjing Decoction can inhibit the inflammatory response in rats with desmoplastic male dry eye disease, and to explore its potential mechanism of action. Methods　60 healthy male SD rats were randomly divided into 4 groups：sham surgery group of 15, negative control group of 15, positive control group of 15, Huashi Running Decoction group of 15) . The sham operated group rats only underwent abdominal incision without removing both testicles and epididymis；Rats in the negative control group, positive control group and Huashi Runjing Decoction group were removed both testicles and epididymis. After 1 week of modeling, the model was validated by fluorescein staining (FL), break-up time (BUT), and tear secretion test (SIT) . After the wound healed, the rats were given the appropriate drug interventions. The degree of corneal breakage, tear film rupture time and tear secretion of rats in each group were detected by FL method, BUT method and SIT method respectively after 8 weeks of drug administration. The levels of COX-2, TNF-α and IL-6 in the serum of each group of rats were observed by ELISA; HE staining was used to observe the pathomorphological changes in the lacrimal gland tissue of rats in each group. The expression of key inflammatory factors COX-2, TNF-α and IL-6 in the lacrimal gland tissues of rats in each group was observed by immunohistochemistry and Western blot. Results　Compared with the sham-operated group, the negative control group, positive control group and Huashi Runjing Decoction group showed an increase in the area of sodium fluorescein positive staining in the cornea, a significant increase in FL score, a shortening of tear film rupture time and a decrease in tear secretion, an increase in serum COX-2, TNF-α and IL-6 levels, atrophy of the lacrimal gland tissue vesicles, an irregular shape, a disorganized structure, a decrease in intracytoplasmic zymogen particles, and a large number of inflammatory cell infiltrations. The expression levels of inflammatory factors COX-2, TNF-α and IL-6 increased in the tissues ( P < 0.05) ; Compared with the negative control group, the Huashi Runjing Decoction group showed a significant decrease in the area of sodium fluorescein positive staining, a significant decrease in FL score, a significant increase in tear film rupture time and tear secretion, a decrease in serum COX-2, TNF-α and IL-6 levels, a decrease in atrophy of the lacrimal gland tissue vesicles, a more regular shape, a clearer structure, an increase in the number of intracytoplasmic zymogen particles, an increase in inflammatory cell infiltration and a decrease in the area of lacrimal gland tissue. The expression levels of COX-2, TNF-α and IL-6 in lacrimal gland tissues were reduced (P < 0.05) . Conclusion　Huashi Runjing Decoction could reduce the protein expression of COX-2, TNF-α and IL-6 in the lacrimal gland tissue of castrated male dry eye disease rats, and inhibit the inflammatory response. [ABSTRACT FROM AUTHOR]

Objective　 To analyze the clinical features of adult hemophagocytic syndrome （HPS） and the correlation between the levels of various cytokines and prognosis. Methods　 The data of 19 adult patients with hemophagocytic syndrome were collected, and the clinical features and laboratory tests were analyzed. The blood samples were collected for 12 cytokine levels detection, and the correlation between the levels and prognosis was analyzed. Results　The levels of cytokines IL-1β, IL-2, IL-4, IL-8, IL-10, IL-6, IFN-γand TNF-α in HPS patients were higher than those in non-HPS non-infected patients （P < 0.05）. The levels of IL-4, IL-5, IL10, IFN-γ and TNF-α in HPS patients were higher than those in non-HPS patients （P < 0.05）, the ROC curve of IL-10, IFN-γ and TNF-α showed that the AUC of IL-10, IFN-γ and TNF-α was between 0.7 and 0.9, indicating accuracy in the diagnosis of HPS. Fib was significantly lower than that in the survival group （P < 0.05）, while there were no significant differences in age, sex, clinical features （fever, liver, spleen, lymphadenopathy） and laboratory tests （ALT, AST, TBIL, ALB, Fer, TG, ACN, HGB, PLT） between the HPS death group and the survival group （P > 0.05）, and the levels of IL-1β, IL-8 and TNF-α in HPS death group were significantly higher than those in survival group （P < 0.05）. Conclusion　HPS may present increased levels of various cytokines, among which IL-10, IFN-γ and TNF-α may be significant for the diagnosis of HPS. The significant decrease of Fib and the significant increase of IL-1β, IL-8 and TNF-α may indicate a poor prognosis. [ABSTRACT FROM AUTHOR]