1. In Vitro Transformation by Bovine Papillomavirus
- Author
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Israel Dvoretzky, Douglas R. Lowy, Sisir K. Chattopadhyay, and Yasuharu Nakabayashi
- Subjects
Genes, Viral ,viruses ,Dermatology ,Biology ,Transfection ,medicine.disease_cause ,Biochemistry ,DNA sequencing ,Cell Line ,Mice ,chemistry.chemical_compound ,Extrachromosomal DNA ,medicine ,Animals ,Cloning, Molecular ,Papillomaviridae ,Molecular Biology ,Bovine papillomavirus 1 ,Bovine papillomavirus ,Mutation ,DNA Restriction Enzymes ,Cell Biology ,Cell Transformation, Viral ,biology.organism_classification ,Molecular biology ,Long terminal repeat ,chemistry ,Genetic Code ,DNA, Viral ,Chromosome Deletion ,In vitro recombination ,DNA - Abstract
We have studied tumorigenic transformation of mouse tissue culture cells by bovine papillomavirus (BPV) as a model of papillomavirus-induced cell proliferation. When BPV or its 7.9-kb full-length viral DNA genome induces focal transformation of mouse calls, the viral DNA is maintained in the transformed cells as multiple extrachromosomal copies. The transforming capacity was initially localized to a 69% subgenomic fragment of the viral DNA genome. We have further characterized the BPV DNA sequences that can encode the transforming function by generating and analyzing the transforming activity of a series of BPV DNA deletion mutants. The results indicated that two discontinuous segments of the viral DNA are required for transformation. One segment, near the 5' end of the 69% transforming fragment, probably represents a control element of the viral DNA. The second segment, which lies within the 3' end of the 69% fragment, encodes transforming sequences of the viral DNA. A retroviral control element (the long terminal repeat DNA. A retroviral control element (the long terminal repeat DNA of Harvey murine sarcoma virus) will activate the 2.3-kb segment at the 3' end of the 69% fragment to transform the mouse cells.
- Published
- 1984
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