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Silver Oxide nanoparticles (AgONPs) have been revealed to have many applications, including antibacterial behavior. However, the conventional methods for synthesizing AgO NPs often use toxic chemicals that may be detrimental to human health and environmental factors. The crystalline structure and size of obtained AgO NPs were confirmed by Powder X-ray diffraction analysis with 35–70 nm. Functional groups have been identified in Fourier transform infrared spectroscopy. The result of Energy Dispersive X-ray Spectroscopy confirmed the presence of the elements in the synthesized AgO NPs. The energy band gap is determined using UV–visible spectroscopy. Morphological examination of prepared AgO NPs, comprising spherical and rod-like structures, was performed using a Transmission, and scanning electron microscope. The antibacterial activity was evaluated using Gram-negative (Klebsiella pneumonia, Escherichia coli) and Gram-positive (Staphylococcus aureus, Bacillus cereus) bacteria from Agar Well Diffusion Method. The results show that the green synthesis of AgO NPs have better antibacterial activity. The inhibition zone diameter were observedfor all microorganisms. The green synthesis of AgO NPs from herbal plant leaf extracts is a promising method for developing safe and effective antibacterial agents. [ABSTRACT FROM AUTHOR]