1. Dengue Virus Ribonucleic Acid Detection Rates in Blood Donors Correlate With Local Infection Incidences During a Dengue Outbreak in Taiwan.
- Author
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Chen, Yun-Yuan, Lu, Chi-Te, Tsai, Mei-Hua, Yang, Cheng-Fen, Shu, Pei-Yun, Wu, Chia-Wen, Chen, Jen-Wei, Hung, Chi-Ming, Wei, Sheng-Tang, Hou, Sheng-Mou, and Chen, Pei-Jer
- Subjects
DENGUE hemorrhagic fever ,DENGUE viruses ,RNA ,DENGUE ,BLOOD donors ,NUCLEIC acids ,IMMUNOGLOBULINS ,FLAVIVIRUSES ,DISEASE incidence ,EPIDEMICS ,RESEARCH funding ,VIRAL antibodies - Abstract
Background: Evidence for mitigation of transfusion-transmitted dengue informed by surveillance data is lacking. In this study, we evaluated the risk of positive dengue viral (DENV) ribonucleic acid (RNA) from blood transfusions during a large outbreak in Taiwan.Methods: Serum collected from blood donors living in districts experiencing the dengue epidemic were tested for DENV RNA using a qualitative transcription-mediated nucleic acid amplification assay (TMA). The TMA-reactive specimens were further tested for immunoglobulin (Ig)M and IgG antibodies, nonstructural protein 1 (NS1) antigen, and viral RNA by reverse-transcription polymerase chain reaction. We estimated DENV RNA prevalence and the number of DENV infections among blood donors.Results: A total of 4976 specimens were tested for DENV RNA, and 21 were TMA-reactive. The detection rate was 0.84 (95% confidence interval [CI], 0.15-4.73), 3.36 (95% CI, 1.31-8.60), and 6.19 (95% CI, 3.14-12.17) per 1000 donors in districts where the weekly dengue incidence was 5-50, 50-200, and 200 or more per 100 000 residents, respectively. Alanine aminotransferase screening only detected 4.4% of TMA-reactive donations. A total of 143 transfusion-transmitted DENV infections probably occurred during this outbreak, accounting for 9.2 in 10 000 dengue infections.Conclusions: Approximately 0.5%-1% of blood donations were DENV RNA positive in epidemic districts. The correlation of DENV RNA rates with dengue incidence may inform the design of effective control measures. [ABSTRACT FROM AUTHOR]- Published
- 2022
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