1. Variations of bacterial 16S rDNA phylotypes prior to and after chlorination for drinking water production from two surface water treatment plants
- Author
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Jean-Baptiste Poitelon, Eric Prestel, Bénédicte Welté, Michael Dubow, Michel Joyeux, Jean-Pierre Duguet, Institut de génétique et microbiologie [Orsay] (IGM), and Université Paris-Sud - Paris 11 (UP11)-Centre National de la Recherche Scientifique (CNRS)
- Subjects
Fresh Water ,010501 environmental sciences ,01 natural sciences ,Applied Microbiology and Biotechnology ,Ferric Compounds ,MESH: Charcoal ,MESH: Disinfection ,MESH: Water Purification ,RNA, Ribosomal, 16S ,MESH: Ecosystem ,MESH: Phylogeny ,MESH: Water Supply ,Betaproteobacteria ,Phylogeny ,0303 health sciences ,MESH: DNA, Ribosomal ,biology ,MESH: Paris ,Biodiversity ,6. Clean water ,MESH: RNA, Ribosomal, 16S ,[SDV.MP]Life Sciences [q-bio]/Microbiology and Parasitology ,Environmental chemistry ,Charcoal ,MESH: Fresh Water ,Water treatment ,MESH: Ferric Compounds ,Proteobacteria ,Chlorine ,Water Microbiology ,Biotechnology ,DNA, Bacterial ,Paris ,chemistry.chemical_element ,Bioengineering ,DNA, Ribosomal ,MESH: Biodiversity ,Microbiology ,Water Purification ,03 medical and health sciences ,Chlorides ,Water Supply ,[SDV.BBM]Life Sciences [q-bio]/Biochemistry, Molecular Biology ,MESH: Chlorine ,MESH: Chlorides ,Ribosomal DNA ,Ecosystem ,030304 developmental biology ,0105 earth and related environmental sciences ,Bacteria ,Alphaproteobacteria ,Ribosomal RNA ,biology.organism_classification ,MESH: DNA, Bacterial ,MESH: Water Microbiology ,Disinfection ,MESH: Bacteria ,chemistry ,MESH: Filtration ,Surface water ,Filtration - Abstract
International audience; We examined the variations of bacterial populations in treated drinking water prior to and after the final chlorine disinfection step at two different surface water treatment plants. For this purpose, the bacterial communities present in treated water were sampled after granular activated carbon (GAC) filtration and chlorine disinfection from two drinking water treatment plants supplying the city of Paris (France). Samples were analyzed after genomic DNA extraction, polymerase chain reaction (PCR) amplification, cloning, and sequencing of a number of 16S ribosomal RNA (rRNA) genes. The 16S rDNA sequences were clustered into operational taxonomic units (OTUs) and the OTU abundance patterns were obtained for each sample. The observed differences suggest that the chlorine disinfection step markedly affects the bacterial community structure and composition present in GAC water. Members of the Alphaproteobacteria and Betaproteobacteria were found to be predominant in the GAC water samples after phylogenetic analyses of the OTUs. Following the chlorine disinfection step, numerous changes were observed, including decreased representation of Proteobacteria phylotypes. Our results indicate that the use of molecular methods to investigate changes in the abundance of certain bacterial groups following chlorine-based disinfection will aid in further understanding the bacterial ecology of drinking water treatment plants (DWTPs), particularly the disinfection step, as it constitutes the final barrier before drinking water distribution to the consumer's tap.
- Published
- 2009
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