1. Cutting edge: STAT activation by IL-19, IL-20 and mda-7 through IL-20 receptor complexes of two types.
- Author
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Dumoutier L, Leemans C, Lejeune D, Kotenko SV, and Renauld JC
- Subjects
- Carrier Proteins metabolism, Cell Line, Genes, Reporter, Genes, Tumor Suppressor, Humans, Macromolecular Substances, Models, Biological, Receptors, Interleukin genetics, STAT3 Transcription Factor, Signal Transduction, Transcriptional Activation, Transfection, Interleukin-22, DNA-Binding Proteins metabolism, Growth Substances pharmacology, Interleukin-10 pharmacology, Interleukins pharmacology, Receptors, Cell Surface, Receptors, Interleukin metabolism, Trans-Activators metabolism
- Abstract
IL-10-related cytokines include IL-20 and IL-22, which induce, respectively, keratinocyte proliferation and acute phase production by hepatocytes, as well as IL-19, melanoma differentiation-associated gene 7, and AK155, three cytokines for which no activity nor receptor complex has been described thus far. Here, we show that mda-7 and IL-19 bind to the previously described IL-20R complex, composed by cytokine receptor family 2-8/IL-20Ralpha and DIRS1/IL-20Rbeta (type I IL-20R). In addition, mda-7 and IL-20, but not IL-19, bind to another receptor complex, composed by IL-22R and DIRS1/IL20Rbeta (type II IL-20R). In both cases, binding of the ligands results in STAT3 phosphorylation and activation of a minimal promoter including STAT-binding sites. Taken together, these results demonstrate that: 1) IL-20 induces STAT activation through IL-20R complexes of two types; 2) mda-7 and IL-20 redundantly signal through both complexes; and 3) IL-19 signals only through the type I IL-20R complex.
- Published
- 2001
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