1. A Novel Neutralizing Antibody Specific to the DE Loop of VP1 Can Inhibit EV-D68 Infection in Mice.
- Author
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Huiwen Zheng, Jingjing Wang, Bingxiang Li, Lei Guo, Heng Li, Jie Song, Zening Yang, Hongzhe Li, Haitao Fan, Xing Huang, Haiting Long, Chen Cheng, Manman Chu, Zhanlong He, Wenhai Yu, Jiaqi Li, You Gao, Ruotong Ning, Nan Li, and Jinxi Yang
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ENTEROVIRUSES , *CRANIAL nerve diseases , *RESPIRATORY diseases , *RHESUS monkeys , *SIALIC acids , *PNEUMONIA , *IMMUNOGLOBULINS - Abstract
Enterovirus D68 (EV-D68) belongs to the picornavirus family and was first isolated in CA, USA, in 1962. EV-D68 can cause severe cranial nerve system damage such as flaccid paralysis and acute respiratory diseases such as pneumonia. There are currently no efficient therapeutic methods or effective prophylactics. In this study, we isolated the mAb A6-1 from an EV-D68-infected rhesus macaque (Macaca mulatta) and found that the Ab provided effective protection in EV-D68 intranasally infected suckling mice. We observed that A6-1 bound to the DE loop of EV-D68 VP1 and interfered with the interaction between the EV-D68 virus and a2,6-linked sialic acids of the host cell. The production of A6-1 and its Ab properties present a bridging study for EV-D68 vaccine design and provide a tool for analyzing the process by which Abs can inhibit EV-D68 infection. [ABSTRACT FROM AUTHOR]
- Published
- 2018
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