1. An improved, ultrasensitive method for the detection of IgM oligoclonal bands in cerebrospinal fluid
- Author
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E.J Thompson, C.A.W. Rijcken, and A.W Teelken
- Subjects
Immunofixation ,Oligoclonal band ,IgM ,Immunology ,Immunoglobulins ,CSF ,Cross Reactions ,Intrathecal ,Sensitivity and Specificity ,Fixatives ,Cerebrospinal fluid ,Immunology and Allergy ,Humans ,oligoclonal band ,Electrophoresis, Agar Gel ,agarose electrophoresis ,Chromatography ,biology ,Chemistry ,Oligoclonal Bands ,immunofixation ,Membranes, Artificial ,Molecular biology ,Immunoglobulin M ,Immunoglobulin G ,Agarose gel electrophoresis ,Polyvinyldifluoride ,IMMUNOGLOBULIN-M ,biology.protein ,Immunofixation technique ,Polyvinyls - Abstract
A new, 10-fold more sensitive method, based on an improved immunofixation technique, has been devised to detect oligoclonal IgM bands in unconcentrated cerebrospinal fluid (CSF). Using agarose gel electrophoresis, 5 mu l of an unconcentrated sample containing oligoclonal bands was separated and blotted on to a polyvinyldifluoride membrane. To visualise the pattern, a peroxidase-labelled double-antibody technique was used. No prior concentration of CSF was needed and the process required only 5 h. The technique may prove very useful in diagnosing an early intrathecal immune response.
- Published
- 1997
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