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Your search keyword '"A. J. L. Hudson"' showing total 2 results
Start Over Author "A. J. L. Hudson" Journal journal of histochemistry & cytochemistry
2 results on '"A. J. L. Hudson"'

1. Nonparametric flow cytometry analysis

Author

George L. Irvin, J L Hudson, and C B Bagwell

Subjects
Normalization (statistics), Histology, Channel (digital image), Cytological Techniques, Statistics as Topic, Lymphocyte Activation, Flow cytometry, Photometry, Bayes' theorem, Histogram, medicine, Humans, Lymphocytes, Phytohemagglutinins, Cells, Cultured, Statistical hypothesis testing, Mathematics, medicine.diagnostic_test, business.industry, Nonparametric statistics, Pattern recognition, DNA, Artificial intelligence, Anatomy, business, Smoothing
Abstract

A nonparametric statistical test for the analysis of flow cytometry derived histograms is presented. The method involves smoothing and translocation of data, area normalization, channel by channel determination of the mean and S.D., and use of Bayes' theorem for unknown histogram classification. With this statistical method, different sets of histograms from numerous biological systems can be compared.

Published
1979
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2. Multiparameter kinetic analysis of killer cell initiation by using immune RNA

Author

C B Bagwell, P J Kmieck, George L. Irvin, and J L Hudson

Subjects
Cytotoxicity, Immunologic, Histology, Cytological Techniques, Cell, Spleen, Biology, Tritium, Cell Line, Photometry, Mice, chemistry.chemical_compound, Immune system, medicine, Animals, Lymphocytes, RNA, Neoplasm, Cytotoxicity, Leukemia, Experimental, Cell Cycle, RNA, Cell cycle, Molecular biology, Cell biology, medicine.anatomical_structure, chemistry, Cell culture, Anatomy, Thymidine
Abstract

By treating nonsensitized C57BL/6J spleen derived lymphocytes with EL-4 tumor cell directed xenogeneic extracted RNA we were able to monitor early changes in cellular DNA content by flow cytometric (FCM) analysis and 3H-thymidine uptake. These kinetic parameters were correlated with cell mediated cytotoxicity which appeared as early as 8 hr after activation as measured by release of chromium-51 from labeled EL-4 target cells. Flow cytometric analysis and 3H-thymidine uptake data shown peak S phase activity at 72 hr. Maximum cytotoxicity was observed at 48 hr. Cell cycle kinetic parameters were correlated with the appearance of cell mediated cytotoxicity.

Published
1979
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