1. Chromosomal Mapping of 18S-28S rRNA Genes and 10 cDNA Clones of Human Chromosome 1 in the Musk Shrew (Suncus murinus)
- Author
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J. K. Seong, J. S. Masangkay, Rea Victoria P. Anunciado, V.-B. Dang, Takahiro Yamagata, N. Nomura, Akira Ishikawa, K. Tanaka, Yoichi Matsuda, Takahiro Nagase, Takao Namikawa, A. Kuroiwa, and Kazumi Matsubara
- Subjects
Heterozygote ,DNA, Complementary ,Biology ,Complementary DNA ,28S ribosomal RNA ,RNA, Ribosomal, 28S ,RNA, Ribosomal, 18S ,Genetics ,Homologous chromosome ,medicine ,Animals ,Humans ,Molecular Biology ,Gene ,In Situ Hybridization, Fluorescence ,Genetics (clinical) ,Chromosomes, Human, Pair 14 ,medicine.diagnostic_test ,Chromosomes, Human, Pair 10 ,Shrews ,Brain ,Chromosome Mapping ,Chromosome ,Evolution of mammals ,Suncus ,biology.organism_classification ,Chromosome Banding ,Chromosomes, Human, Pair 1 ,DNA Probes ,Chromosomes, Human, Pair 7 ,Biotechnology ,Fluorescence in situ hybridization - Abstract
The direct R-banding fluorescence in situ hybridization (FISH) method was used to map 18S-28S ribosomal RNA genes and 10 human cDNA clones on the chromosomes of the musk shrew (Suncus murinus). The chromosomal locations of 18S-28S ribosomal RNA genes were examined in the five laboratory lines and wild animals captured in the Philippines and Vietnam, and the genes were found on chromosomes 5, 6, 9, and 13 with geographic variation. The comparative mapping of 10 cDNA clones of human chromosome 1 demonstrated that human chromosome 1 consisted of at least three segments homologous to Suncus chromosomes (chromosomes 7, 10, and 14). This approach with the direct R-banding FISH method is useful for constructing comparative maps between human and insectivore species and for explicating the process of chromosomal rearrangements during the evolution of mammals.
- Published
- 2001
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