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Your search keyword '"Gonzalez-Gallego J"' showing total 26 results
Start Over Author "Gonzalez-Gallego J" Journal journal of hepatology
26 results on '"Gonzalez-Gallego J"'

1. SAT-146 - Molecular characterization of autophagic and apoptotic signaling induced by Sorafenib in liver cancer cells: In vitro and in vivo studies

Author

Rodríguez-Hernández, M.Á., González, R., De la Rosa, Á.J., Gallego, P., Contreras, L., Ordóñez, R., Navarro-Villarán, E., Rodríguez-Arribas, M., González-Gállego, J., Álamo-Martínez, J.M., Gómez, L.M.M., Castillo, J.A.D.C., Quiles, J.L., Fuentes, J.M., De la Cruz, J., Mauriz, J.L., Padillo, F.J., and Muntané, J.

Published
2018
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26. Hepatitis C virus NS5A and core proteins induce oxidative stress-mediated calcium signalling alterations in hepatocytes.

Author

Dionisio N, Garcia-Mediavilla MV, Sanchez-Campos S, Majano PL, Benedicto I, Rosado JA, Salido GM, and Gonzalez-Gallego J

Subjects
Actins metabolism, Antioxidants pharmacology, Calcium Signaling drug effects, Calcium-Binding Proteins pharmacology, Cell Line, Cell Survival physiology, Cysteine Proteinase Inhibitors pharmacology, Cytokines metabolism, Hepacivirus physiology, Hepatitis C drug therapy, Hepatitis C metabolism, Hepatocytes cytology, Hepatocytes drug effects, Homeostasis drug effects, Homeostasis physiology, Humans, Oxidative Stress drug effects, Quercetin pharmacology, Reactive Nitrogen Species metabolism, Reactive Oxygen Species metabolism, Virus Replication drug effects, Virus Replication physiology, Calcium metabolism, Calcium Signaling physiology, Hepatocytes physiology, Oxidative Stress physiology, Viral Core Proteins physiology, Viral Nonstructural Proteins physiology
Abstract

Background/aims: The hepatitis C virus (HCV) structural core and non-structural NS5A proteins induce in liver cells a series of intracellular events, including elevation of reactive oxygen and nitrogen species (ROS/RNS). Since oxidative stress is associated to altered intracellular Ca(2+) homeostasis, we aimed to investigate the effect of these proteins on Ca(2+) mobilization in human hepatocyte-derived transfected cells, and the protective effect of quercetin treatment., Methods: Ca(2+) mobilization and actin reorganization were determined by spectrofluorimetry. Production of ROS/RNS was determined by flow cytometry., Results: Cells transfected with NS5A and core proteins showed enhanced ROS/RNS production and resting cytosolic Ca(2+) concentration, and reduced Ca(2+) concentration into the stores. Phenylephrine-evoked Ca(2+) release, Ca(2+) entry and extrusion by the plasma membrane Ca(2+)-ATPase were significantly reduced in transfected cells. Similar effects were observed in cytokine-activated cells. Phenylephrine-evoked actin reorganization was reduced in the presence of core and NS5A proteins. These effects were significantly prevented by quercetin. Altered Ca(2+) mobilization and increased calpain activation were observed in replicon-containing cells., Conclusions: NS5A and core proteins induce oxidative stress-mediated Ca(2+) homeostasis alterations in human hepatocyte-derived cells, which might underlie the effects of both proteins in the pathogenesis of liver disorders associated to HCV infection.

Published
2009
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