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Over 5000 public drinking water systems in the US are out of compliance with the Lead and Copper Rule. Lead leaching from lead pipes is limited by the solubility of a naturally occurring scale. Changes in water quality may cause this scale to become more soluble, releasing lead into the drinking water. We propose a novel electrochemical method to prevent lead leaching from lead pipes. In this method, an aluminum wire and an alkaline phosphate electrolyte are inserted into the pipes. The pipes are then anodized for 2 h by using an external power supply, resulting in the electrodeposition of an insoluble aluminum oxide layer on top of the preexisting scale. This technology was tested on lead pipes from the EBMUD water distribution systems located in Berkeley, CA, using recirculating synthetic and actual tap water for 120 days. The untreated pipes leached an average of 23 ppb and 38 ppb of lead respectively, when using free chlorine and monochloramine as disinfection residuals. In contrast, the treated pipes leached 3 ppb and 5 ppb respectively. These results suggest that the proposed treatment has the potential to prevent lead leaching regardless of the disinfection residual and thus should be further explored in a field trial.

There is a long-standing debate over the effectiveness of chemical extraction methods in assessing soil metal phytoavailability. This study addresses the limitations of widely-used chemical extraction methods and presents the water-extractable pool as a more reliable indicator based on wheat pot experiments using homogenized agricultural soil amended with lime materials, phosphate, and biochar. Over 120 days' pot experiments, Cd accumulation in whole wheat plants and tissues exhibited positive relationships with water-extractable Cd concentrations at heading and maturity stage (Spearman's rho: 0.521–0.851; P < 0.05), revealing that the water-extractable pool instead of other pools better indicates wheat metal accumulation. Water-extractable metal concentrations are effective in assessing phytoavailability of metals primarily in ionic forms in soil solution (e.g, Zn, Cd), but less reliable for metals strongly complexed with dissolved organic matter (DOM) or sensitive to redox conditions. It demonstrated that water-extractable metal concentrations and chemical forms are key factors, fundamentally determined by metal properties and impacted by environmental factors. This study clarifies a more direct link between chemical extraction and plant metal uptake mechanisms. Given the extensive application of chemical extraction methods over several decades, this study will help advance soil metal risk assessment and remediation practices. [Display omitted] • The water-extractable pool is more reliable for metal phytoavailability assessment. • Water-extractable Cd concentrations at heading and maturity stages significantly correlated with wheat accumulation. • Water-extractable metal concentrations and forms are key factors determining phytoavailability. [ABSTRACT FROM AUTHOR]

Cadmium (Cd) pollution has gained significant attention in mangrove sediments due to its high toxicity and mobility. However, the sources of Cd and the factors influencing its accumulation in these sediments have remained elusive. In this study, we utilized lead (Pb) isotopic signatures for the first time to assess Cd contamination in mangrove sediments from the northern region of the Beibu Gulf. A strong correlation was observed between Cd and Pb concentrations in the mangrove sediments, suggesting a shared source that can be estimated using Pb isotopic signatures. By employing a Bayesian mixing model, we determined that 70.1 ± 8.2 % of Cd originated from natural sources, while 12.9 ± 4.9 %, 9.8 ± 3.7 %, and 7.1 ± 3.4 % were attributed to agricultural activities, non-ferrous metal smelting, and coal combustion, respectively. Our study clearly suggests that natural Cd could also dominate the high Cd content. Agricultural activities were the most important anthropogenic Cd sources, and the increased anthropogenic Cd accumulation in mangrove sediment was related to organic matter. This study introduces a novel approach for assessing Cd contamination in mangrove sediment, providing useful insights into Cd pollution in coastal wetlands. [Display omitted] • Cd and Pb concentrations in mangrove sediment were significantly correlated. • Source contribution of Cd was estimated by Pb isotopic signatures. • Natural source can dominate the Cd content in the mangrove sediment. • Elevated anthropogenic Cd accumulation in mangrove sediment was related to organic matter. [ABSTRACT FROM AUTHOR]

The resource of trace lead (Pb2+) from wastewater bearing intricate components is imperative for sustainable progression of the lead-acid battery industry. Herein, we fabricated a tannic acid-based covalent polymeric hydrogel (TA@PMAM) with antimicrobial properties and stability via facile Michael addition reaction. The incorporation of tannic acid (TA) through robust covalent bond leads to a stable porous 3D covalent polymer network with almost no loss of mechanical properties even after 20 compression cycles. Batch adsorption experiments of TA@PMAM revealed an extraordinary adsorption capacity of Pb2+(Qe =196.6 mg/g), achieving 87.2 % of Pb2+ adsorption within the first 5 min owing to porous structure, numerous adsorption sites and good hydrophilicity. Moreover, TA@PMAM demonstrated a strong affinity for Pb2+ in the presence of the interfere metal ions (Cu2+, Co2+, Mn2+ etc.) due to the carbonyl and phenolic hydroxyl that can specifically pair with Pb2+. Stable adsorption properties of TA@PMAM were confirmed in fixed bed column adsorption experiment using lead-acid batteries wastewater, retaining 79.56 % of initial adsorption capacity even after 10 times' reuse. Besides, TA@PMAM possesses a broad spectrum of antimicrobial properties. This study sheds novel light on the design and fabrication of adsorbent, which holds great potential for commercialization in recovering lead from battery industrial wastewater. [Display omitted] • Stable tannic acid-based covalent crosslinked 3D network is facilely fabricated. • High selectivity of Pb2+ is due to its specific pairing with carbonyl and hydroxyl. • TA@PMAM features anti-interference, reusability and broad-spectrum antimicrobial. • Calculations and cycling experiments confirm the robust attachment of TA. [ABSTRACT FROM AUTHOR]

Both sulfur (S) supply and legume-rhizobium symbiosis can significantly contribute to enhancing the efficiency of phytoremediation of heavy metals (HMs). However, the regulatory mechanism determining the performance of legumes at lead (Pb) exposure have not been elucidated. Here, we cultivated black locust (Robinia pseudoacacia L.), a leguminous woody pioneer species at three S supply levels (i.e., deficient, moderate, and high S) with rhizobia inoculation and investigated the interaction of these treatments upon Pb exposure. Our results revealed that the root system of Robinia has a strong Pb accumulation and anti-oxidative capacity that protect the leaves from Pb toxicity. Compared with moderate S supply, high S supply significantly increased Pb accumulation in roots by promoting the synthesis of reduced S compounds (i.e., thiols, phytochelatin), and also strengthened the antioxidant system in leaves. Weakened defense at deficient S supply was indicated by enhanced oxidative damage. Rhizobia inoculation alleviated the oxidative damage of its Robinia host by immobilizing Pb to reduce its absorption by root cells. Together with enhanced Pb chelation in leaves, these mechanisms strengthen Pb detoxification in the Robinia -rhizobia symbiosis. Our results indicate that appropriate S supply can improve the defense of legume-rhizobia symbiosis against HM toxicity. [Display omitted] • High S supply significantly increased Pb accumulation in roots by promoting the synthesis of reduced S compounds. • High S strengthened the antioxidant system in leaves of Robinia under Pb. • Weakened defense at deficient S supply indicated by enhanced oxidative damage. • Root of Robinia has a strong Pb accumulation and anti-oxidative capacity that protect the leaves from Pb toxicity. • Rhizobia inoculation alleviated the oxidative damage of its Robinia host by immobilizing Pb to reduce its absorption in roots. [ABSTRACT FROM AUTHOR]

The use of sewage sludge as a soil improver has been promoted in agroecosystems. However, sludges can contain toxic trace elements because of suboptimal wastewater treatment. Nonetheless, field studies investigating the negative effects of these practices on pollinators are lacking. We collected honeybees from an area where sewage sludge use is widespread, and one where it is precluded. Trace elements in soils and bees were quantified. Cadmium, chromium, lead, mercury, and nickel were investigated because they were the least correlated elements to each other and are known to be toxic. Their levels were related to oxidative stress and energy biomarkers, midgut epithelial health, body size and wing asymmetry of honeybees. We found increased carbohydrate content in sites with higher cadmium levels, increased histological damage to the midgut epithelium in the sewage sludge area, and the presence of dark spherites in the epithelium of bees collected from the sites with the highest lead levels. Finally, we found that honeybees with the highest lead content were smaller, and that wing fluctuating asymmetry increased in sites with increasing levels of mercury. To the best of our knowledge, this is the first comprehensive study of the concentration and effects on honeybees of trace elements potentially deriving from soil amendment practices. [Display omitted] • Sewage sludges used as soil improver in agroecosystems can contain trace elements. • This is one of the first studies to use an integrated approach to investigate this issue. • We sampled honeybees from two areas with different sewage sludge usage. • We found negative effects of trace elements on morpho-physiological endpoints. • First evidence of adverse effects of sewage sludge use under field conditions. [ABSTRACT FROM AUTHOR]

High-ash coal slime-based silica fertilizer (CSF) has the potential to provide mineral nutrients and passivate lead (Pb) in the soil to ensure the sustainable development of the coal industry and agriculture. This study investigated the performance and passivation mechanism of CSF, which contains potassium tobermorite and potassium silicate as the main components for soil improvement. Leaching experiments showed that low-crystalline muscovite was the only crystalline phase for CSF etching and that the silicon (Si), calcium (Ca), and potassium (K) in CSF had significant citric solubility. Soil cultivation and planting trials confirmed the ability of CSF to neutralize soil acidity, increase available soil Si and K, improve exchangeable Ca content, reduce the bioefficacy of Pb (exchangeable Pb by 19–75 % and carbonate-bound Pb by 6–18 %), and increase residual state Pb content. Compared to untreated Pb-contaminated soil, the 0.4 % CSF treatment reduced Pb in Chinese cabbage (Brassica rapa) by 25 % and increased plant biomass, Ca, and K by 37 %, 36 %, and 4 %, respectively. At the same time, soil pH increased by 0.58, and residual state Pb increased by 5 %. In CSF-treated soils, lead silicate is the dominant form of Pb present in the residual state. First-principle calculations showed that Pb 3 Si 2 O 7 (cohesion energy −1.98 eV) formed by the passivation of Pb by CSF had greater stability in the soil compared to lead carbonate (PbCO 3) (cohesion energy −1.38 eV) and lead sulfate (PbSO 4) (cohesion energy −1.41 eV). This work shows the promising application of coal slime mineral fertilizers prepared using hydrothermal methods for soil improvement. [Display omitted] • High-ash coal slime-based silica fertilizer (CSF) was used to remediate soil. • CSF contains effective Si, Ca, and K that remain active for a long time. • CSF reduces the mobility and availability of Pb by increasing Pb residual content. • CSF effectively inhibits the uptake of Pb by plants. • xPbO·ySiO 2 is more stable and has a greater cohesive energy than PbSO 4 and PbCO 3. [ABSTRACT FROM AUTHOR]

The long-term effects of low-level, chronic exposure to lead and cadmium through ingestion are often overlooked, despite the urgency surrounding the clinical onset and worsening of certain pathologies caused by these metals. This work reviews current legislation, global ingestion levels, and blood levels in the general population to emphasize the need for reactivity towards this exposure, especially in at-risk populations, including patients with early-stage renal and chronic kidney disease. Global data indicates persistent chronic ingestion of lead and cadmium, with no decreasing trend in recent years, and a daily consumption of tens of micrograms worldwide. Moreover, the average blood lead and cadmium levels in the general population are concerning in many countries with some significantly exceeding healthy limits, particularly for children. Technologies developed to cleanse soil and prevent heavy metal contamination in food are not yet applicable on a global scale and remain financially inaccessible for many communities. Addressing this chronic ingestion at the human level may prove more beneficial in delaying the onset of associated clinical pathologies or preventing them all together. [Display omitted] • Lead and cadmium ingestion is the main source of exposure in the general population. • Observed plateau of daily ingestion of tens of micrograms since 2010s. • Concerning global blood lead and cadmium levels among healthy adults and children. • At-risk patients show increased susceptibility to lead and cadmium ingestion. • No existing gold-standard response to heavy metal ingestion. [ABSTRACT FROM AUTHOR]

Lead (Pb) poisoning is estimated to account for 1 % of the global disease burden. The gold standard for diagnosing lead poisoning in human body relies on blood lead level (BLL), which is always performed in hospitals using expensive instruments. However, there are still many countries and regions with a lack of medical resources (without enough professional medical staff and analytical instruments). To achieve a facile diagnosis of lead poisoning by ordinary residents (without any expertise), this study conducted a research study on 810 participants to discover and validate a new lead poisoning indicator (creatinine-corrected urinary lead level, cULL) beyond BLL in non-invasive samples. A point-of-care testing (POCT) device to measure cULL was developed, equipped with liquid-phase microextraction and electromembrane extraction on a paper-based analytical device for on-site separation of lead and creatinine in the urine, using a smartphone for the quantification of analytes. The cULL as a novel indicator and the POCT device developed could be effective in reducing the risk of damage from lead contamination. [Display omitted] ● cULL is used as a novel non-invasive indicator for lead poisoning beyond BLL. ● EME and LPME are integrated for simultaneous separation of lead and creatinine. ● LOD values for lead and creatinine are 0.4 μg/L and 0.04 g/L, respectively. ● The indicator cULL has a sensitivity of 95.74 % for diagnosis of lead poisoning. [ABSTRACT FROM AUTHOR]

Glaciers are considered secondary sources of pollutants, including radioisotopes such as Cesium or Plutonium, with heightened concentrations compared to other ecosystems. The predicted melting of glaciers poses a substantial risk of releasing stored radioisotopes, yet understanding the glacier-specific factors influencing their concentration remains limited. This study investigates the relationship between glacier altitude, surface area, organic matter content in dark supraglacial sediment (cryoconite), precipitation, and activity concentrations of natural (210Pb) and anthropogenic radionuclides (137Cs and 241Am) across 19 Alpine glaciers. Results indicate that radioisotope concentrations depend on organic matter content in the cryoconite, highlighting the role of biotic-abiotic interactions in pollutant accumulation on glaciers. Moreover, 210Pb activity concentration decreases with glacier altitude, likely due to atmospheric variations in 222Rn. Water precipitation events, such as during peaks in 137Cs deposition and after the Chernobyl Nuclear Power Plant disaster, do not impact current activity concentrations. Importantly, radioisotope concentrations in cryoconite are higher on smaller glaciers. This directly supports the hypothesis that the cryoconite retains a significant share of radioisotopes stored in the ice during intensive melting. Since many small glaciers in the Alps are predicted to disappear within the next 50 years, we anticipate release of radioisotopes to mountain ecosystems might be higher than previously forecasted. [Display omitted] • 210Pb, 137Cs and 241Am concentrate on small glaciers during the melting of ice. • Small glaciers that are predicted to melt are a threat to glacier-adjacent biota. • Accumulation of radioisotopes on glaciers is not spatially related. • 210Pb accumulates stronger in lower-elevated glaciers. [ABSTRACT FROM AUTHOR]

Microplastics (MPs) have attracted widespread attention because they can lead to combined toxicity by adsorbing heavy metals from the environment. Exposure to lead (Pb), a frequently adsorbed heavy metal by MPs, is common. In the current study, the coexistence of MPs and Pb was assessed in human samples. Then, mice were used as models to examine how co-exposure to MPs and Pb promotes aortic medial degeneration. The results showed that MPs and Pb co-exposure were detected in patients with aortic disease. In mice, MPs and Pb co-exposure promoted the damage of elastic fibers, loss of vascular smooth muscle cells (VSMCs), and release of inflammatory factors. In vitro cell models revealed that co-exposure to MPs and Pb induced excessive reactive oxygen species generation, impaired mitochondrial function, and triggered PANoptosome assembly in VSMCs. These events led to PANoptosis and inflammation through the cAMP/PKA-ROS signaling pathway. However, the use of the PKA activator 8-Br-cAMP or mitochondrial ROS scavenger Mito-TEMPO improved, mitochondrial function in VSMCs, reduced cell death, and inhibited inflammatory factor release. Taken together, the present study provided novel insights into the combined toxicity of MPs and Pb co-exposure on the aorta. [Display omitted] • MPs and high levels of Pb exposure were detected in patients with aortic disease. • Co-exposure to MPs and Pb promotes aortic medial degeneration in mice. • Co-exposure to MPs and Pb induces PANoptosis and inflammation in VSMCs. • The cAMP/PKA-ROS signaling pathway is involved in co-exposure to MPs and Pb. [ABSTRACT FROM AUTHOR]

Lead (Pb) pollution in sediments remains a major concern for ecosystem quality due to the robust interaction at the sediment/water interface, particularly in shallow lakes. However, understanding the mechanism behind seasonal fluctuations in Pb mobility in these sediments is lacking. Here, the seasonal variability of Pb concentration and isotopic ratio were investigated in the uppermost sediments of a shallow eutrophic drinking lake located in southeast China. Results reveal a sharp increase in labile Pb concentration during autumn-winter period, reaching ∼ 3-fold higher levels than during the spring-summer seasons. Despite these fluctuations, there was a notable overlap in the Pb isotopic signatures within the labile fraction across four seasons, suggesting that anthropogenic sources are not responsible for the elevated labile Pb concentration in autumn-winter seasons. Instead, the abnormally elevated labile Pb concentration during autumn-winter was probably related to reduction dissolution of Fe/Mn oxides, while declined labile Pb concentration during spring-summer may be attributed to adsorption/precipitation of Fe/Mn oxides. These large seasonal changes imply the importance of considering seasonal effects when conducting sediment sampling. We further propose a solution that using Pb isotopic signatures within the labile fraction instead of the bulk sediment can better reflect the information of anthropogenic Pb sources. [Display omitted] • Larger, 3-fold, seasonal fluctuations were observed in the labile Pb concentration. • Pb sources were not responsible for the elevated labile Pb concentration during autumn-winter seasons. • Fe/Mn oxides dominated the seasonal variations of labile Pb concentration. • Algal bloom can increase Pb mobility in sediments. [ABSTRACT FROM AUTHOR]

Lead halide perovskite has demonstrated remarkable potential in the wearable field due to its exceptional photoelectric conversion capability. However, its lead toxicity issue has consistently been subject to criticism, significantly impeding its practical application. To address this challenge, an innovative approach called lead-rivet was proposed for the in-situ growth of perovskite crystalline structures. Through the formation of S-Pb bonds, each Pb2+ ion was firmly immobilized on the surface of the silica matrix, enabling in situ growth of perovskite nanocrystals via ion coordination between Cs+ and halide species. The robust S-Pb bonding effectively restricted the mobility of lead ions and stabilized the perovskite structure without relying on surface ligands, thereby not only preventing toxicity leakage but also providing a favorable interface for depositing protective shells. The obtained perovskites exhibit intense and narrow-band fluorescence with full-width at half-maximum less than 23 nm and show excellent stability to high temperature (above 202 °C) and high humidity (water immersion over 27 days), thus making it possible to be used in varies textile technologies including melt spinning and wet spinning. The lead leakage rate of particles is only 4.15 % demonstrating excellent toxicity inhibition performance. The prepared fibers maintained good extensibility and flexibility which could be used for 3D-printing and textiles weaving. Most importantly, the detected Pb2+ leaching was negligible as low as to 0.732 ppb which meet the standard of World Health Organization (WHO) for drinking water (<10 ppb), and the cell survival rate remained 99.196 % for PLA fluorescent filament after 24 h cultivation which showing excellent safety to human body and environment. This study establishes a controllable and highly adaptable synthesis method, thereby providing a promising avenue for the safe utilization of perovskite materials. [Display omitted] • Lead-rivet strategy was proposed for in-situ crystallization of perovskites. • S-Pb bonds can stabilize the perovskites with better heat stability more than 202 °C. • More than 96 % lead pollution in perovskite could be prevented by lead-rivet strategy. • Continuous fluorescent filaments were achieved by melt-spinning and wet-spinning. [ABSTRACT FROM AUTHOR]

Lake Nansi, primarily dominated by macrophytes, faces threats from heavy metals and antibiotics due to human activity. This study investigated sediment dissolved organic matter (SDOM) characteristics and complexation of lead (Pb) and tetracycline (TC) in barren zone (BZ) and submerged macrophytes zone (PZ). Additionally, a microbial degradation experiment was conducted to examine its impact on the regional variations in complexation. SDOM abundance and protein-like materials in PZ was significantly greater than in BZ, indicating a probable contribution from the metabolism and decomposition of submerged macrophytes. Both zones exhibited a higher affinity of SDOM for Pb compared to TC, with all four components participating in Pb complexation. Protein-like materials in PZ had a higher binding ability (Log K Pb =4.19 ± 1.07, Log K TC =3.89 ± 0.67) than in BZ (Log K Pb =3.98 ± 0.61, Log K TC =3.69 ± 0.13), suggesting a potential presence of organically bound Pb and TC due to the higher abundance of protein-like materials in PZ. Although microbial communities differed noticeably, the degradation patterns of SDOM were similar in both zones, affecting the binding ability of SDOM in each. Notably, the fulvic-like component C4 emerged as the dominant binding material for both Pb and TC in both zones. Degradation might increase the amount of organically bound TC due to the increase in the Log K TC. [Display omitted] • Effect of heterogeneity and biodegradation on DOM complexation was conducted. • Submerged macrophytes zone (PZ) possessed a higher protein-like material abundance. • Lead (Pb) and tetracycline (TC) were selected to explore complexation difference. • PZ possessed higher binding ability rather than barren zone (BZ) for Pb and TC. • Degradation enhanced binding ability of fulvic-like material for both Pb and TC. [ABSTRACT FROM AUTHOR]

Compared to traditional lead-remediating materials, natural-occurring paleosol is ubiquitous and could be a promising alternative due to its rich content in calcite, a substance known for its lead-removal ability via carbonate dissolution-PbCO 3 precipitation process. Yet, the capability of paleosol to remediate aqueous solutions polluted with heavy metals, lead included, has rarely been assessed. To fill this gap, a series of column permeation experiments with influent Pb2+ concentrations of 2000, 200, and 20 mg/L were conducted and monitored by the spectral induced polarization technique. Meanwhile, the SEM-EDS, XRD, XPS, FTIR and MIP tests were carried out to unveil the underlying remediation mechanisms. The Pb-retention capacity of paleosol was 1.03 mmol/g. The increasing abundance of Pb in the newly-formed crystals was confirmed to be PbCO 3 by XRD, SEM-EDS and XPS. Concurrently, after Pb2+ permeation, the decreasing calcite content in paleosol sample from XRD test, and the appearance of Ca2+ in the effluent confirmed that the dissolution of CaCO 3 followed by the precipitation of PbCO 3 was the major mechanism. The accumulated Pb (i.e., the diminished Ca) in paleosol was inversely proportional (R2 >0.82) to the normalized chargeability (m n), an SIP parameter denoting the quantity of polarizable units (primarily calcite). [Display omitted] • Introduced paleosol for effective Pb2+ remediation. • Employed SIP for real-time, non-intrusive monitoring. • Revealed dissolution of CaCO 3 and PbCO 3 precipitation as key mechanisms. • Demonstrated significant SIP correlation with Pb2+ adsorption. • Highlighted SIP's potential in environmental remediation monitoring. [ABSTRACT FROM AUTHOR]

In this study, we investigated the impact of fluctuating water levels on the distribution of lead (Pb) and zinc (Zn) in soil and sediments at a historical Pb-Zn smelting site along the Xiangjiang River. Despite the high pH levels (7 to 11) in the study area, which generally inhibits heavy metal solubility, we found that regular changes in water levels still affect Pb-Zn movement. Soil analysis revealed distinct redox zones within the unconfined aquifer, as shown by the variable Fe/Mn and Ce/Ce* ratios. Advanced techniques such as Mn K-edge XAFS, Mössbauer spectroscopy, and TOF-SIMS indicated persistent Fe-Mn redox cycling and highlighted the presence of Pb and Zn-rich manganese oxides near sulfur-bearing minerals. These findings suggest that acidic microzones produced by the oxidation of sulfur-bearing minerals become "refuges" for microbial and heavy metal activity. Considering that sulfur-containing minerals are widespread waste types in nonferrous metal smelting sites, these findings are instructive for a better understanding of the transformation mechanisms of heavy metal ions in nonferrous metal smelting-polluted environments and for guiding pollution remediation strategies. [Display omitted] • Active biogeochemical cycling still occurs in soils with a pH of up to 11. • Seasonal water level fluctuations can still drive the Fe-Mn redox cycle in highly alkaline environments. • Surface oxidation of sulfur-containing particles provides a microenvironment for the movement of heavy metals. [ABSTRACT FROM AUTHOR]

There are many heavy metal stresses in agricultural biological systems, especially cadmium (Cd) stress, which prevent the full growth of plants, lead to a serious decline in crop yield, and endanger human health. Molybdenum (Mo), an essential nutrient element for plants, regulates plant growth mainly by reducing the absorption of heavy metals and protecting plants from oxidative damage. The aim of this study was to determine the protective effect of Mo (1 μM) application on wheat plants under conditions of Cd (10 μM) toxicity. The biomass, Cd and Mo contents, photosynthesis, leaf and root ultrastructure, antioxidant system, and active oxygen content of the wheat plants were determined. Mo increased the total chlorophyll content of wheat leaves by 43.02% and the net photosynthetic rate by 38.67%, and ameliorated the inhibitory effect of cadmium on photosynthesis by up-regulating photosynthesis-related genes and light-trapping genes. In addition, Mo reduced the content of superoxide anion (O 2 •—) by 16.55% and 31.12%, malondialdehyde (MDA) by 20.75% and 7.17%, hydrogen peroxide (H 2 O 2) by 24.69% and 8.17%, and electrolyte leakage (EL) by 27.59% and 16.82% in wheat leaves and roots, respectively, and enhanced the antioxidant system to reduce the burst of reactive oxygen species and alleviate the damage of Cd stress on wheat. According to the above results, Mo is considered a plant essential nutrient that enhances Cd tolerance in wheat by limiting the absorption, accumulation and transport of Cd and by regulating antioxidant defence mechanisms. Cadmium (Cd)，is one of the most toxic heavy metals in the environment, and Cd pollution is a global environmental problem that threatens food security and human health. Molybdenum (Mo), as an essential plant nutrient, is often used to resist environmental stress. However, the mechanism of Mo treatment on wheat subjected to Cd stress has not been reported. In this study, we systematically analysed the effects of Mo on the phenotype, physiology, biochemistry, ultrastructure and Cd content of wheat subjected to Cd stress, and comprehensively analysed the transcriptomics. It not only reveals the mechanism of Mo tolerance to Cd stress in wheat, but also provides new insights into phytoremediation and plant growth in Cd-contaminated soil. [Display omitted] • Mo mitigates the inhibitory effect of Cd on wheat seedling growth. • Mo up-regulates photosynthesis and light-trapping related genes. • Mo reduces the burst of reactive oxygen species by enhancing the antioxidant system of wheat under Cd stress. • Mo reduces Cd uptake and maintains ion homeostasis by regulating the expression of Cd related transport genes. [ABSTRACT FROM AUTHOR]

Heavy metal pollution at an abandoned smelter pose a significant risk to environmental health. However, remediation strategies are constrained by inadequate knowledge of the polymetallic distribution, speciation patterns, and transformation factors at these sites. This study investigates the influence of soil minerals, heavy metal occurrence forms, and environmental factors on heavy metal migration behaviors and speciation transformations. X-ray diffraction analysis revealed that the minerals associated with heavy metals are mainly hematite, franklinite, sphalerite, and galena. Sequential extraction results suggest that lead and zinc are primarily present in the organic-sulfide fractions (F4) and residual form (F5) in the soil, accounting for over 70% of the total heavy metal content. Zinc displayed greater instability in carbonate-bound (16%) and exchangeable (2%) forms. The migration and diffusion patterns of heavy metals in the subsurface environment were visualized through the simulation of labile state heavy metals, demonstrating high congruence with groundwater pollution distribution patterns. The key environmental factors influencing heavy metal stable states (F4 and F5) were assessed by integrating random forest models and redundancy analysis. Primary factors facilitating Pb transformation into stable states were available phosphorus, clay content, depth, and soil organic matter. For Zn, the principal drivers were Mn oxides, soil organic matter, clay content, and inorganic sulfur ions. These findings enhance understanding of the distribution and transformation of heavy metal speciation and can provide valuable insights into controlling heavy metal pollution at non-ferrous smelting sites. [Display omitted] • The samples from the site reveal considerable heavy metal contamination due to zinc iron spinel, galena, sphalerite, hematite. • Pb and Zn in the soil exist primarily in the stable form, with the labile state closely aligns with groundwater contamination. • The zinc content contributes to its excessive presence in groundwater due to temporal and spatial accumulation. • Available phosphorus, Mn oxides, clay, depth, and soil organic matter facilitate the transformation of heavy metals into stable states. [ABSTRACT FROM AUTHOR]

Lead is a neurotoxic contaminant that exists widely in the environment. Although lead neurotoxicity has been found to be tightly linked to gut microbiota disturbance, the effect of host metabolic disorders caused by gut microbiota disturbance on lead neurotoxicity has not been investigated. In this work, the results of new object recognition tests and Morris water maze tests showed that chronic low-dose lead exposure caused learning and memory dysfunction in mice. The results of 16 S rRNA sequencing of cecal contents and fecal microbiota transplantation showed that the neurotoxicity of lead could be transmitted through gut microbiota. The results of untargeted metabolomics and bile acid targeted metabolism analysis showed that the serum bile acid metabolism profile of lead-exposed mice was significantly changed. In addition, supplementation with TUDCA or INT-777 significantly alleviated chronic lead exposure-induced learning and memory impairment, primarily through inhibition of the NLRP3 inflammasome in the hippocampus to relieve neuroinflammation. In conclusion, our findings suggested that dysregulation of host bile acid metabolism may be one of the mechanisms of lead-induced neurotoxicity, and supplementation of specific bile acids may be a possible therapeutic strategy for lead-induced neurotoxicity. [Display omitted] • Chronic lead exposure disrupts gut microbiota associated host bile acid metabolic homeostasis. • TUDCA and INT-777 supplementation alleviate lead-induced learning and memory impairment. • TUDCA and INT-777 attenuate lead-induced neuroinflammation by inhibiting NLRP3 inflammasome. [ABSTRACT FROM AUTHOR]

The critical challenge of effectively removing Pb-EDTA complexes and Pb(II) ions from wastewater is pivotal for environmental remediation. This research introduces a cutting-edge bulk-MoS 2 /H 2 O 2 system designed for the simultaneous decomplexation of Pb-EDTA complexes and extraction of free Pb(II) ions, streamlining the process by eliminating the need for subsequent treatment stages. The system exhibits outstanding efficiency, achieving 98.1% decomplexation of Pb-EDTA and 98.6% removal of Pb. Its effectiveness is primarily due to the generation of reactive oxygen species, notably •OH and O 2 •- radicals, facilitated by bulk-MoS 2 and H 2 O 2. Key operational parameters such as reagent dosages, Pb(II): EDTA molar ratios, solution pH, and the presence of coexisting ions were meticulously evaluated to determine their impact on the system's performance. Through a suite of analytical techniques, the study confirmed the disruption of Pb–O and Pb–N bonds, further elucidating the decomplexation process. It also underscored the synergistic role of bulk-MoS 2 's adsorption properties and the formation of PbMoO 4 -like precipitates in enhancing Pb elimination. Demonstrating the bulk-MoS 2 /H 2 O 2 system as a robust, one-step solution that meets stringent Pb emission standards, this study provides in-depth insights into the removal mechanisms of Pb-EDTA, affirming its potential for broader application in wastewater treatment practices. [Display omitted] • Our system exhibited over 98% efficiency in Pb-EDTA decomplexation and Pb removal. • The study showed robust performance and adaptability under various conditions. • Mechanistic insights revealed the roles of •OH and O 2 •– in Pb-EDTA breakdown. • The dual mechanism of adsorption and precipitation in Pb removal was elucidated. [ABSTRACT FROM AUTHOR]

This study employs a combination of bibliometric and epidemiological methodologies to investigate the relationship between metal exposure and glucose homeostasis. The bibliometric analysis quantitatively assessed this field, focusing on study design, predominant metals, analytical techniques, and citation trends. Furthermore, we analyzed cross-sectional data from Beijing, examining the associations between 14 blood metals and 6 glucose homeostasis markers using generalized linear models (GLM). Key metals were identified using LASSO-PIPs criteria, and Bayesian kernel machine regression (BKMR) was applied to assess metal mixtures, introducing an "Overall Positive/Negative Effect" concept for deeper analysis. Our findings reveal an increasing research interest, particularly in selenium, zinc, cadmium, lead, and manganese. Urine (27.6%), serum (19.0%), and whole blood (19.0%) were the primary sample types, with cross-sectional studies (49.5%) as the dominant design. Epidemiologically, significant associations were found between 9 metals—cobalt, copper, lithium, manganese, nickel, lead, selenium, vanadium, zinc—and glucose homeostasis. Notably, positive-metal mixtures exhibited a significant overall positive effect on insulin levels, and notable interactions involving nickel were identified. These finding not only map the knowledge landscape of research in this domain but also introduces a novel perspective on the analysis strategies for metal mixtures. [Display omitted] • Innovative integration of bibliometrics and epidemiological analysis. • Provides quantified review data informing epidemiological practices. • Enhanced the mixture analysis strategy. • Introducing Overall Positive/Negative Effects concepts. [ABSTRACT FROM AUTHOR]

Modern metallurgical and smelting activities discharge the lead-containing wastewater, causing serious threats to human health. Bacteria and urease applied to microbial-induced carbonate precipitation (MICP) and enzyme-induced carbonate precipitation (EICP) are denatured under high Pb2+ concentration. The nano-hydroxyapatite (nHAP)-assisted biomineralization technology was applied in this study for Pb immobilization. Results showed that the extracellular polymers and cell membranes failed to secure the urease activity when subjected to 60 mM Pb2+. The immobilization efficiency dropped to below 50% under MICP, whereas it due to a lack of extracellular polymers and cell membranes dropped to below 30% under EICP. nHAP prevented the attachment of Pb2+ either through competing with bacteria and urease or promoting Ca2+/Pb2+ ion exchange. Furthermore, CO 3 2- from ureolysis replaced the hydroxyl (-OH) in hydroxylpyromorphite to encourage the formation of carbonate-bearing hydroxylpyromorphite of higher stability (Pb 10 (PO 4) 6 CO 3). Moreover, nHAP application overcame an inability to provide nucleation sites by urease. As a result, the immobilization efficiency, when subjected to 60 mM Pb2+, elevated to above 80% under MICP-nHAP and to some 70% under EICP-nHAP. The findings highlight the potential of applying the nHAP-assisted biomineralization technology to Pb-containing water bodies remediation. [Display omitted] • nHAP adsorbed Pb2+ by competing with the bacteria to prevent their attachment. • nHAP encouraged Ca2+/Pb2+ ion exchange to prevent their attachment as well. • Carbonate-bearing hydroxylpyromorphite reduced Pb migration potential. • Lead apatite to be wrapped by cerussite reduced Pb migration potential as well. [ABSTRACT FROM AUTHOR]

Plant metal uptake can occur through both soil-root and atmospheric transfer from leaves. The latter holds potential implications for development of biofiltration systems. To explore this potential, it is crucial to understand entrapment capacity and metal sources within plants. As ferns absorb materials from atmosphere, this study focuses on two abundant fern species growing in densely populated and highly polluted regions of Eastern India. Gravimetric quantification, elemental concentration and Pb isotopic analyses were performed by segregating the ferns into distinct components: foliage dusts (loose dust (LD) and wax-bound dust (WD)) and plant tissue (leaves and roots). To understand metal sources, the study analyzes soil, and atmospheric particulates (PM 10 and dust fall (DF)). Results indicate that, while LDs have soil dust influence, wax entraps atmospheric particulates and translocates them inside the leaves. Furthermore, roots demonstrate dissimilar isotopic ratios from soil, while displaying close association with atmospheric particulates. Isotopic composition and subsequent mixing model reveal dominant contribution from DF in leaves (53–73%) and roots (33–86%). Apart from DF, leaf Pb is sourced from PM 10 (21–38%) with minimal contribution from soil (6–10%). Conversely, in addition to dominance from DF, roots source Pb primarily from soil (12–62%) with a meagre 2–8% contribution from PM 10. [Display omitted] • Mitigation of PM bound heavy metals is essential to prevent global fatalities. • Studying PM capture capacity and sources in ferns help design remediation process. • Pb isotopes and elemental concentration prove fern tissues capture atmospheric PM. • Ferns hold potential to improve air quality, implying their practical use. [ABSTRACT FROM AUTHOR]

Biochar, an environmentally friendly material, was found to passivate lead (Pb) in contaminated soil effectively. This study utilized spectroscopic investigations and partial least squares path modeling (PLS-PM) analysis to examine the impact of coconut-fiber biochar (CFB) on the translocation, accumulation, and detoxification mechanisms of Pb in soil-rice systems. The results demonstrated a significant decrease (p < 0.05) in bioavailable Pb concentration in paddy soils with CFB amendment, as well as reduced Pb concentrations in rice roots, shoots, and brown rice. Synchrotron-based micro X-ray fluorescence analyses revealed that CFB application inhibited the migration of Pb to the rhizospheric soil region, leading to reduced Pb uptake by rice roots. Additionally, the CFB treatment decreased Pb concentrations in the cellular protoplasm of both roots and shoots, and enhanced the activity of antioxidant enzymes in rice plants, improving their Pb stress tolerance. PLS-PM analyses quantified the effects of CFB on the accumulation and detoxification pathways of Pb in the soil–rice system. Understanding how biochar influences the immobilization and detoxification of Pb in soil–rice systems could provide valuable insights for strategically using biochar to address hazardous elements in complex agricultural settings. [Display omitted] • Coconut-fiber biochar (CFB) immobilized Pb and reduced its uptake by rice plants. • CFB can inhibit Pb accumulation in rhizosphere soil, rice root, and brown rice. • CFB reduced Pb concentration in rice plants at the subcellular level. • CFB enhanced antioxidant enzymes and improved Pb tolerance of rice plants. • PLS-PM quantified the CFB effect on Pb transport and mechanisms in the system. [ABSTRACT FROM AUTHOR]

Heavy metals (HMs) are common contaminants with major concern of severe environmental and health problems. This study evaluated the effects of organo-mineral amendments (mesquite biochar (MB), zeolite (ZL) and bentonite (BN) alone and in combination) applied at different rates to promote the maize (Zea mays L.) growth by providing essential nutrient and improving the soil physio-chemical properties under zinc (Zn) and lead (Pb) contamination. Result revealed that the incorporation of organo-mineral amendments had significantly alleviated Pb and Zn contamination by maize plants and improved the physiological and biochemical attributes of plants. Combined application of organo-mineral amendments including BMA-1, BMA-2 and BMA-3 performed excellently in terms of reducing Pb and Zn concentrations in both leaves (19–60%, 43–75%, respectively) and roots (24–59%, 42–68%, respectively) of maize. The amendments decreased the extractable, reducible, oxidisable and residual fractions of metals in soil and significantly reduced the soil DTPA-extractable Pb and Zn. BMA-1 substantially improved antioxidant enzyme activities in metal-stressed plants. This study indicated that combined use of organo-mineral amendments can effectively reduce the bioavailability and mobility of Pb and Zn in co-contaminated soils. Combined application of organo-mineral amendments could be viable remediation technology for immobilization and metal uptake by plants in polluted soils. [Display omitted] • Biochar, zeolite and bentonite significantly reduced soil DTPA extractable Pb and Zn. • Biochar and zeolite reduced translocation, biological concentration, H 2 O 2 and proline contents. • Bentonite showed better reduction in the concentration of Pb and Zn in maize crop. • Combined use of amendments was effective in reducing metal uptake by plants than alone application. [ABSTRACT FROM AUTHOR]

Particle size is a critical influencing factor in assessing human exposure risk as fine particles are generally more hazardous than larger coarse particles. However, how particle composition influences human health risk is only poorly understood as different studies have different utilised different definitions and as a consequence there is no consensus. Here, with a new methodology taking insights of each size fraction load (%GSF load), metal bioaccessibility, we classify which specific particle size can reliably estimate the human exposure risk of lead and other metals. We then validate these by correlating the metals in each size fraction with those in human blood, hair, crop grain and different anthropogenic sources. Although increasing health risks are linked to metal concentration these increase as particle size decrease, the adjusted-risk for each size fraction differs when %GSF load is introduced to the risk assessment program. When using a single size fraction (250–50 µm, 50–5 µm, 5–1 µm, and < 1 µm) for comparison, the risk may be either over- or under-estimated. However, by considering bulk and adjusting the risk, it would be possible to obtain results that are closer to the real scenarios, which have been validated through human responses and evidence from crops. Fine particle size fractions (< 5 µm) bearing the mineral crystalline or aggregates (CaCO 3 , Fe 3 O 4 , Fe 2 O 3 , CaHPO 4 , Pb 5 (PO 4) 3 Cl) alter the accumulation, chemical speciation, and fate of metals in soil/dust/sediment from the different sources. Loaded lead in the size fraction of < 50 µm has a significantly higher positive association with the risk-receptor biomarkers (BLLs, Hair Pb, Corn Pb, and Crop Pb) than other size fractions (bulk and 50–250 µm). Thus, we conclude that the < 50 µm fraction would be likely to be recommended as a reliable fraction to include in a risk assessment program. This methodology acts as a valuable instrument for future research undertakings, highlighting the importance of choosing suitable size fractions and attaining improved accuracy in risk assessment results that can be effectively compared. [Display omitted] • A novel risk-assessment model based on grain size fraction load and bioaccessibility is defined. • Higher loading of metal in fine size fractions always have an anthropogenic origin. • Micro-nano size fraction bearing crystalline, or aggregate alter metal accumulation and speciation. • The forming of aggregates has strong associated with the anthropogenic emissions. • A <50 µm would be recommend as a reliable fraction for risk assessment program. [ABSTRACT FROM AUTHOR]

The adsorption behavior and interaction mechanisms of extracellular polymeric substances (EPS) of Pseudomonas aeruginosa OMCS-1 towards chromium (Cr), lead (Pb), and cadmium (Cd) were investigated. EPS-covered (EPS-C) cells exhibited significantly higher (p < 0.0001; two-way ANOVA) removal of Cr (85.58 ± 0.39%), Pb (81.98 ± 1.02%), and Cd (73.88 ± 1%) than EPS-removed (EPS-R) cells. Interactions between EPS-heavy metals were spontaneous (ΔG<0). EPS-Cr(VI) and EPS-Pb(II) binding were exothermic (ΔH<0), while EPS-Cd(II) binding was endothermic (ΔH>0) process. EPS bonded to Pb(II) via inner-sphere complexation by displacement of surrounding water molecules, while EPS-Cr(VI) and EPS-Cd(II) binding occurred through outer-sphere complexation via electrostatic interactions. Increased zeta potential of Cr (29.75%), Pb (41.46%), and Cd (46.83%) treated EPS and unchanged crystallinity (CI XRD =0.13), inferred EPS-metal binding via both electrostatic interactions and complexation mechanism. EPS-metal interaction was predominantly promoted through hydroxyl, amide, carboxyl, and phosphate groups. Metal adsorption deviated EPS protein secondary structures. Strong static quenching mechanism between tryptophan protein-like substances in EPS and heavy metals was evidenced. EPS sequestered heavy metals via complexation with C-O, C-OH, C O/O-C-O, and NH/NH 2 groups and ion exchange with –COOH group. This study unveils the fate of Cr, Pb, and Cd on EPS surface and provides insight into the interactions among EPS and metal ions for metal sequestration. [Display omitted] • The presence of EPS on bacterial surface facilitates the adsorption of metal ions. • EPS-metal binding was spontaneous, exothermic for Cr and Pb, and endothermic for Cd. • Electrostatic interaction and complexation mechanism of EPS and metal binding. • Heavy metal adsorption deviated the secondary structures of EPS proteins. • Static quenching mechanism between tryptophan containing proteins and heavy metals. [ABSTRACT FROM AUTHOR]

The mass balance of reconstituted Cd, Cu, Pb and Zn fluxes from 1683 to 2021 was compared to the current levels of the soil used only for vegetable production in the King's Kitchen Garden in Versailles (France). This comparison was made on the basis of 4 scenarios of organic matter application in the 18th and 19th centuries and by an uncertainty analysis over the entire period. The topsoil contamination falls within that of French kitchen gardens. Modelling of past fluxes predicted the correct trend (an increase) and order of magnitude of the soil metal contents. It produced a relatively accurate evaluation of the Cu and Zn contents. The model underestimated the Pb contents by about 80%, revealing a large and unknown source of soil contamination by this metal. The calculation overestimated the current Cd levels by about 100%, probably due to various biases, for example on atmospheric fallout or the composition of organic amendments. This assessment shows that modelling the mass balance of trace metal fluxes can be used to predict the long-term trend in the levels of these elements in cultivated soils, providing the input data are chosen according to realistic scenarios. [Display omitted] • A mass balance model for Cd, Cu, Pb and Zn fluxes in cultivated soil was evaluated. • The model predicted the increase in the 4 metal contents in topsoil after 339 years. • The Cu and Zn contents were correctly predicted. • The underestimation of Pb content is attributed to unknown contamination sources. • The overestimation of Cd content seems multicausal. [ABSTRACT FROM AUTHOR]

The soil near tailings areas is relatively barren and contaminated by multi-metal(loid)s, seriously threatening the safety of crop production. Here, biochar and nano-hydroxyapatite (nHAP) were combined to improve the sterilized and unsterilized polymetallic contaminated soil, and soil incubation and soybean pot experiments were designed. Results showed that biochar and nHAP not only increased soil C, N, and P but also effectively reduced multi-metal bioavailability, wherein the combined application of the two amendments had the best effect on metal immobilization. The synergistic effect of the two amendments decreased the acid-soluble contents of Co, Cu, Fe, and Pb in rhizosphere soils up to 86.75%, 80.69%, 89.09%, and 96.70%, respectively. The ameliorant reduced the accumulation of metal(loid)s in soybean plants, and rhizosphere microorganisms inhibited the migration of soil metals to plants. Additionally, biochar and nHAP regulated the rhizosphere soil microbial community. The rhizosphere soil of the sterilization group tended to prioritize the restoration of the original dominant bacteria. As, Pb, Fe, Urease, OM, TN, and TP were the critical environmental variables affecting rhizosphere soil bacterial communities. Therefore, combining biochar and nHAP is an environmentally friendly strategy to reduce polymetallic mobility in tailings soil and crops and improve soil microbial community structure., Competing Interests: Declaration of Competing Interest The authors declare that they have no known competing financial interests or personal relationships that could have appeared to influence the work reported in this paper., (Copyright © 2024 Elsevier B.V. All rights reserved.)

Bioaccessibility and relative bioavailability of As, Cd, Pb and Sb was investigated in 30 legacy gold mining wastes (calcine sands, grey battery sands, tailings) from Victorian goldfields (Australia). Pseudo-total As concentration in 29 samples was 1.45-148-fold higher than the residential soil guidance value (100 mg/kg) while Cd and Pb concentrations in calcine sands were up to 2.4-fold and 30.1-fold higher than the corresponding guidance value (Cd: 20 mg/kg and Pb: 300 mg/kg). Five calcine sands exhibited elevated Sb (31.9-5983 mg/kg), although an Australian soil guidance value is currently unavailable. Arsenic bioaccessibility (n = 30) and relative bioavailability (RBA; n = 8) ranged from 6.10-77.6% and 10.3-52.9% respectively. Samples containing > 50% arsenopyrite/scorodite showed low As bioaccessibility (<20.0%) and RBA (<15.0%). Co-contaminant RBA was assessed in 4 calcine sands; Pb RBA ranged from 73.7-119% with high Pb RBA associated with organic and mineral sorbed Pb and, lower Pb RBA observed in samples containing plumbojarosite. In contrast, Cd RBA ranged from 55.0-67.0%, while Sb RBA was < 5%. This study highlights the importance of using multiple lines of evidence during exposure assessment and provides valuable baseline data for co-contaminants associated with legacy gold mining activities., Competing Interests: Declaration of Competing Interest The authors declare that they have no known competing financial interests or personal relationships that could have appeared to influence the work reported in this paper. The authors declare that generative artificial intelligence (AI) and AI-assisted technologies were not used in the writing process or any other process during the preparation of this manuscript., (Copyright © 2024 The Authors. Published by Elsevier B.V. All rights reserved.)

Pb 2+ is a heavy metal ion pollutant that poses a serious threat to human health and ecosystems. The conventional methods for detecting Pb 2+ have several limitations. In this study, we introduce a novel fluorescent probe that enables the detection of Pb 2+ in the near-infrared region, free from interference from other common ions. A unique characteristic of this probe is its ability to rapidly and accurately identify Pb 2+ through ratiometric measurements accompanied by a large Stokes shift of 201 nm. The limit of detection achieved by probe was remarkably low, surpassing the standards set by the World Health Organization, and outperforming previously reported probes. To the best of our knowledge, this is the first organic small-molecule fluorescent probe with both near-infrared emission and ratiometric properties for the detection of Pb 2+ . We present a triple-mode sensing platform constructed using a probe that allows for the sensitive and selective recognition of Pb 2+ in common food items. Furthermore, we successfully conducted high-quality fluorescence imaging of Pb 2+ in various samples from common edible plants, HeLa cells, Caenorhabditis elegans, and mice. Importantly, the probe-Pb 2+ complex exhibited tumour-targeting capabilities. Overall, this study presents a novel approach for the development of fluorescent probes for Pb 2+ detection., Competing Interests: Declaration of Competing Interest The authors declare that they have no known competing financial interests or personal relationships that could have appeared to influence the work reported in this paper., (Copyright © 2024. Published by Elsevier B.V.)

The simultaneous sensing and remediation of multiple heavy metal ions in wastewater or soil with microorganisms is currently a significant challenge. In this study, the microorganism Bacillus subtilis was used as a chassis organism to construct two genetic circuits for sensing and adsorbing heavy-metal ions. The engineered biosensor can sense three heavy metal ions (0.1-75 μM of Pb 2+ and Cu 2+ , 0.01-3.5 μM of Hg 2+ ) in situ real-time with high sensitivity. The engineered B. subtilis TasA-metallothionein (TasA-MT) biofilm can specifically adsorb metal ions from the environment, exhibiting remarkable removal efficiencies of 99.5% for Pb 2+ , 99.9% for Hg 2+ and 99.5% for Cu 2+ in water. Furthermore, this engineered strain (as a biosensor and absorber of Pb 2+ , Cu 2+ , and Hg 2+ ) was incubated with biochar to form a hybrid biofilm@biochar (BBC) material that could be applied in the bioremediation of heavy metal ions. The results showed that BBC material not only significantly reduced exchangeable Pb 2+ in the soil but also reduced Pb 2+ accumulation in maize plants. In addition, it enhanced maize growth and biomass. In conclusion, this study examined the potential applications of biosensors and hybrid living materials constructed using sensing and adsorption circuits in B. subtilis, providing rapid and cost-effective tools for sensing and remediating multiple heavy metal ions (Pb 2+ , Hg 2+ , and Cu 2+ )., Competing Interests: Declaration of Competing Interest The authors declare that they have no known competing financial interests or personal relationships that could have appeared to influence the work reported in this paper., (Copyright © 2023 Elsevier B.V. All rights reserved.)

Lead (Pb) and cadmium (Cd) are common heavy metal pollutants that are often found in the soil in soybean agricultural production, adversely impacting symbiotic nitrogen fixation in soybean nodules. In this study, the exposure of soybean nodules to Pb and Cd stress was found to reduce nitrogenase activity. Shifts in the RNA methylation profiles of nodules were subsequently examined by profiling the differential expression of genes responsible for regulating m 6 A modifications and conducting transcriptome-wide analyses of m 6 A methylation profiles under Pb and Cd stress condition. Differentially methylated genes (DMGs) that were differentially expressed were closely related to reactive oxygen species activity and integral membrane components. Overall, 19 differentially expressed DMGs were ultimately determined to be responsive to both Pb and Cd stress, including Glyma.20G082450, which encodes GmAMT1;1 and was confirmed to be a positive regulator of nodules tolerance to Pb and Cd. Together, these results are the first published data corresponding to transcriptome-wide m 6 A methylation patterns in soybean nodules exposed to Cd and Pb stress, and provide novel molecular insight into the regulation of Pb and Cd stress responses in nodules, highlighting promising candidate genes related to heavy metal tolerance, that may also be amenable to application in agricultural production. ENVIRONMENTAL IMPLICATIONS: Lead (Pb) and cadmium (Cd) are prevalent heavy metal pollutants in soil, and pose a major threat to crop production, food security and human health. Here, MeRIP-seq approach was employed to analyze the regulatory network activated in soybean nodules under Pb and Cd stress, ultimately leading to the identification of 19 shared differentially expressed DMGs. When overexpressed, GmATM1;1 was found to enhance the Pb and Cd tolerance of soybean nodules. These results provide a theoretical basis for studies on tolerance to heavy metals in symbiotic nitrogen fixation, and provide an approach to enhancing Pb and Cd tolerance in soybean production., Competing Interests: Declaration of Competing Interest The authors declare that they have no known competing financial interests or personal relationships that could have appeared to influence the work reported in this paper., (Copyright © 2023 Elsevier B.V. All rights reserved.)

Eleven trace metals (Cd, Cr, Fe, Mn, Cu, Ni, Co, Zn, As, Pb, and Ag) in sediments of Bangladesh's ship breaking area were measured by an atomic absorption spectrometer to determine origin, contamination extent, spatial distributions, and associated ecological and human health hazards. This study found considerable quantities of Pb, Cd, Mn, Zn, and Cu when compared with standards and high levels of Pb, Cd, Zn, Cu, As, and Ag contamination according to pollution evaluation indices. Different indices indicate most of the sampling sites were highly polluted. However, spatial distribution maps indicate that trace metals were predominantly deposited in the northern and southern region. The ecological risk index revealed that Cd has the highest while Pb and As had moderate risk. Based on the health index values, Zn for both adults and children were higher than the safe limit while Mn, Pb, Cr, As, Fe, Cu, Ni, and Co for children were close to the threshold. The mean total carcinogenic risk values of Cr, As, and Ni for children and Ni for adults exceeded the permissible threshold. The cancer risk possibilities were further assessed using Monte Carlo simulation. Most trace metals have anthropogenic origins, which were attributed to ship breaking activities., Competing Interests: Declaration of Competing Interest The authors declare that they have no known competing financial interests or personal relationships that could have appeared to influence the work reported in this paper., (Copyright © 2023 Elsevier B.V. All rights reserved.)

Simultaneous quantification of multiple heavy metal ions (HMIs) is essential due to enhanced toxicity induced by synergistic effects. The currently available detection methods suffer from drawbacks such as costly devices, poor anti-interference, and specificity. In this work, a ratiometric electrochemical aptasensor for simultaneous detection of trace lead (Pb 2+ ) and mercury ions (Hg 2+ ) was developed. Here, a metal-organic framework, UiO-66-CNTs, with inherent and stable electrochemical signal was used for loading complementary strands (CSs) and internal reference. Guanine-rich and thymine-rich oligonucleotides, labelled with carbon dots (CDs), acted as aptamers (Apts) and hybridized with CSs to form M-shaped DNA complexes. Pb 2+ and Hg 2+ could be recognized and captured by Apts to form Pb 2+ -G-quadruplex and T-Hg 2+ -T complexes, leading to the destruction of M-shaped DNA complexes and changes in CDs' signal. The current ratios, I CDs /I UiO-66-CNTs , were applied to quantify Pb 2+ and Hg 2+ . Benefiting from the anti-interference ability of the ratiometric strategy and the specificity of Apts, the proposed method enabled detection Pb 2+ and Hg 2+ through simple instrumentation, with detection limits of 2.0 ng mL -1 and 0.5 ng mL -1 . Moreover, it was applied to assess Pb 2+ and Hg 2+ contamination in series of aquatic edible vegetables. The experimental results were consistent with inductively coupled plasma-mass spectrometry (ICP-MS), demonstrating its potential in practical applications., Competing Interests: Declaration of Competing Interest The authors declare that they have no known competing financial interests or personal relationships that could have appeared to influence the work reported in this paper., (Copyright © 2023 Elsevier B.V. All rights reserved.)

Lead (Pb) is present in aquatic environments with a continuous or pulse form due to the regular or irregular discharge of wastewater. These two modes of exposure result in different toxicological effects on aquatic animals. To compare the effects of Pb exposure mode on the swimming behavior of amphibian larvae, this study proposed a combination method to examine the brain-gut axis (gut bacteria, histopathology, metabolomics, and ethology) in order to evaluate the ecotoxic differences in Pelophylax nigromaculatus tadpoles (Gs 21-28) when exposed to continuous (CE100) versus pulse exposure (PE100) of environmental concentrations of Pb (100 μg/L). The results showed that: 1) CE100 significantly decreased the movement distance and swimming activity of the tadpoles compared to PE100 and the control, while there were no significant differences between the control group and PE100. 2) At the phyla level, compared to PE100, CE100 treatment significantly decreased the abundance of Actinobacteria, Firmicutes, Proteobacteria, and Bacteroidetes and increased the abundance of Fusobacteria in the gut. At the genus level, compared to PE100, CE100 significantly increased the abundance of U114 and decreased the abundance of Anaerorhabdus, Exiguobacterium and Microbacterium. 3) Compared to PE100, CE100 changed the metabolites of the brain-gut axis pathway, such as quinolinic acid, L-valine, L-dopa, L-histidine, urocanic acid, L-threonine, γ-aminobutyric acid (GABA), L-glutamate (Glu), acetylcholine (Ach), L-tyrosine (Tyr), L-tryptophan (Trp), and levodopa (DOPA). 4) CE100 and PE100 played a repressive role in the histidine metabolism and tyrosine metabolism pathways and played a promoting role in the purine metabolism and pyrimidine metabolism pathways. This study provides a method for evaluating the toxic effects of heavy metal exposure via two different exposure modes (pulse versus continuous) which tadpoles may encounter in the natural environment from a combined study examining the brain-gut axis., Competing Interests: Declaration of Competing Interest The authors declare that they have no known competing financial interests or personal relationships that could have appeared to influence the work reported in this paper., (Copyright © 2023 Elsevier B.V. All rights reserved.)

Organic amendments are commonly used in assisted phytostabilization of mine wastes by improving their physicochemical and biological properties. These amendments are susceptible to leaching and degradation, resulting in the generation of dissolved organic matter (DOM), which significantly influences the geochemical behaviour of heavy metals (HMs). However, the geochemical behaviour of HMs in metal smelting slag driven by organic amendment-derived DOM remains unclear. In this study, we investigated the impact of cow manure-derived DOM on the release behaviour, bioavailability, and speciation of HMs (Cu, Pb, Zn, and Cd) in zinc smelting slag using a multidisciplinary approach. The results showed that DOM enhanced the weathering of the slag, with a minimal impact on the slag's mineral phases, except for causing gypsum dissolution. The DOM addition resulted in a slight increase in HM release from the slag during the initial inoculation period, followed by a reduction in HM release during the later period. Furthermore, the DOM addition increased the diversity and relative abundance of the bacterial community. This, in turn, led to a decrease in the dissolved organic carbon (DOC) content and enhanced the transformation of labile DOM compounds into recalcitrant compounds. The variation in HM release during various inoculation periods can be attributed to the bacterial decomposition and transformation of DOM, which further enhanced the transformation of HM fractions. Specifically, during the later period, DOM promoted the conversion of a portion of the reducible and oxidizable fractions of Cu, Pb, and Zn into the acid-soluble and residual fractions. Moreover, it partially transformed the reducible, oxidizable, and residual fractions of Cd into the acid-soluble fraction. Overall, this study provides new insights into the geochemical behaviour of HMs in slag governed by the coupling effect of DOM and the bacterial community. These findings have implications for the use of organic amendments in assisted phytostabilization of metal smelting slag. ENVIRONMENTAL IMPLICATION: Metal smelting slag is hazardous due to its high levels of HMs, and its improper disposal has serious consequences for the ecosystem. Organic amendments are employed in assisted phytostabilization of the slag site by improving its microecological properties. However, the impact of organic amendment-derived DOM on HM migration and transformation in slag remains unclear. This study indicated that the coupling effects of DOM and microbes governed the geochemical behaviour of HMs in slag. These findings provide new insights into how organic amendments impact the geochemical behaviour of HMs in slag, contributing to the development of phytostabilization technology., Competing Interests: Declaration of Competing Interest The authors declare that they have no known competing financial interests or personal relationships that could have appeared to influence the work reported in this paper., (Copyright © 2023 Elsevier B.V. All rights reserved.)

Lead (Pb) is a highly toxic element and is not essential to the human body. Lead pollution caused by human activities and a high geological background is considered a global environmental issue. According to the China Geochemical Baseline (CGB) project, the Pearl River Basin had the highest Pb content in alluvial sediments of 30 first-level basins in China. For this reason, it is of great significance to determine the temporal and spatial variations in Pb and their influencing factors in the Pearl River Basin. In this study, 956 stream sediment samples collected in the 1980 s (early stage) and 129 river sediment samples collected from 2008 to 2010 (late stage) were used to study the background value and spatial-temporal variation characteristics of Pb in river sediments in the Pearl River Basin. The Pb source apportionment and an ecological risk assessment were also carried out. The background value of Pb (36.2 mg·kg–1) in the river sediments of the Pearl River Basin was significantly higher than that in China (22.1 mg·kg–1). The parent rocks determine the Pb background in sediments and the high Pb background areas mainly comprised carbonate rocks and acid volcanic rocks. Over 20 years of rapid industrialisation, the average Pb increased from 43.3 to 68.3 mg·kg–1 in the Pearl River Basin. The BCR analysis revealed that Pb mainly existed in the reducible phase (48 % on average) and residue phase (42 % on average). The enrichment factor and geo-accumulation index indicated that the late-stage sediments experienced more Pb pollution than the early-stage sediments. However, the risk assessment code (RAC) showed that there was a low ecological risk of Pb in the late-stage sediments. The factor analysis results for the two rounds of data were significantly different. The Pb content in early-stage sediments was closely related to Al 2 O 3 and Zr, while Pb in the late-stage sediments was mainly related to Zn, As, Sb, Au and Hg, indicating that the increase in Pb in the later samples was mainly influenced by human activities. The Pb isotope composition of the late-stage sediments confirmed that low Pb content was mainly controlled by natural sources, while high Pb content was significantly affected by anthropogenic sources. Combining the results of spatial-temporal variation, chemical speciation and source apportionment indicated that the rapid rise of Pb in late-stage sediments in certain areas could be attributed to mining and smelting activities during the process of industrialisation over 20 years. The anthropogenic exogenous Pb could be immobilised by Fe-Mn (hydro)oxides when it entered the soil, so although there was a high background the ecological risk of Pb in river sediments was low. In the future, Pb pollution control and remediation needs to be strengthened in the Pearl River Basin to avoid the outbreak of potential ecological risks linked to Pb. [Display omitted] • Over 20 years, a significant increase in Pb was observed in Pearl River Basin. • The increase in Pb was attributed to the serious effects of anthropogenic activities. • Pb existed mainly as residue and reducible fractions. • Pb in river sediments was in high background but low ecological risk. [ABSTRACT FROM AUTHOR]

Lead (Pb) and arsenic (As) are prevalent metal contaminants in the environment. Exposures to these metals are associated with impaired neuronal functions and adverse effects on neurodevelopment in children. However, the molecular mechanisms by which Pb and As impair neuronal functions remain poorly understood. Here, we identified F2RL2, TRIM16L , and PANX2 as novel targets of Nuclear factor erythroid 2-related factor 2 (NRF2)—the master transcriptional factor for the oxidative stress response—that are commonly upregulated with both Pb and As in human neural progenitor cells (NPCs). Using a ChIP (Chromatin immunoprecipitation)-qPCR assay, we showed that NRF2 directly binds to the promoter region of F2RL2 , TRIM16L , and PANX2 to regulate expression of these genes. We demonstrated that F2RL2, PANX2 , and TRIM16L have differential effects on cell death, proliferation, and differentiation of NPCs in both the presence and absence of metal exposures, highlighting their roles in regulating NPC function. Furthermore, the analyses of the transcriptomic data on NPCs derived from autism spectrum disorder (ASD) patients revealed that dysregulation of F2RL2 , TRIM16L , and PANX2 was associated with ASD genetic backgrounds and ASD risk genes. Our findings revealed that Pb and As induce a shared NRF2-dependent transcriptional response in NPCs and identified novel genes regulating NPC function. While further in vivo studies are warranted, this study provides a novel mechanism linking metal exposures to NPC function and identifies potential genes of interest in the context of neurodevelopment. [Display omitted] • Pb and As upregulate F2RL2, PANX2, TRIM16L in neural progenitor cells (NPCs). • F2RL2, PANX2, TRIM16L are novel NRF2-dependent genes. • NRF2 directly binds their promotor region. • Knockdown or overexpression of these genes differentially regulate NPC function. • Dysregulation of these genes is linked to autism spectrum disorder gene signature. [ABSTRACT FROM AUTHOR]

In this study, a new lead (Pb) and chlorine (Cl) recovery process via the thermal co-treatment of Municipal solid waste (MSW) incineration fly ash (FA) and waste cathode-ray tubes (CRT) was developed and the synergistic effects under different CRT ratios, temperatures, and residence times were comprehensively investigated. Thermogravimetric experiments revealed that the co-processing of FA and CRT exhibited a remarkable synergistic effect as evidenced by the considerable increase in mass loss and mass-loss rate when compared with the theoretical values. When the mixtures with 50% CRT addition was treated at 1200 °C for 60 min, Pb removal rate reached the maximum value of 98.67%, and the Cl removal rate considerably increased by 37.32% compared to that with FA treatment alone. Additionally, the Cl content in the residue was < 2%. It was mainly attributed to the volatilization of chlorides, such as PbCl 2 , NaCl, and KCl. CaCl 2 generated from the decomposition of CaClOH in FA was conducive to improve Pb removal in CRT through indirect chlorination and destroying the glass structure in CRT. Co-processing of FA and CRT demonstrates promising potential for several benefits, including the reduction in melting temperature, recovery of Pb and Cl from secondary fly ash, and the reutilization of calcium-rich slag. [Display omitted] • Thermal co-treatment of FA and CRT to recover Pb and Cl from the ash was studied. • Adding CRT to FA increased the mass loss and mass loss rate at high temperature. • The addition of CRT reduced the ash fusion temperature. • The Pb and Cl removal rate considerably increased with the addition of 30–50% CRT. • CaCl 2 or CaClOH in FA was the main component to promote Pb volatilization in CRT. [ABSTRACT FROM AUTHOR]

Neither chemical nor physical adsorption play well in heavy metals remediation in acid soil due to the competing behavior of abundant protons, where stable chelators that can be reused are of significant demand. Herein, biochar with abundant nitro and carboxyl groups is prepared, which can be assembled into self-supporting electrode. Under the catalyzation of electricity, the surface decorated -NO 2 on the biochar can be in situ transformed into -NH 2. Combined with the carboxyl group that attached on the same carbon atom, a special α-amino acid-like structure modified biochar (α-AC@BC) can be successfully constructed. Due to the strong affinity between the α-amino acid-like ligand and heavy metals, this α-AC@BC exhibits high removal efficiencies of 83.41%, 80.94%, 92.54% and 77.05% for available copper, cadmium, lead and zinc respectively, even in a strong acid soil with low pH of 4. After four adsorption-desorption cycles, the α-AC@BC could still eliminate 83.88% of copper. The high adsorption energy among -NH 2 , -COOH and heavy metals (−2.99 eV for copper, −1.90 eV for lead, −1.30 eV for zinc and −0.91 eV for cadmium) could form steady coordination structure to guarantee a highly practical application potential of α-AC@BC in strong acid soil. This study provides a novel concept for the decontamination of multiple heavy metal polluted acid soil. [Display omitted] • Constructing a novel α-amino acid-like structure decorated biochar (α-AC@BC). • α-AC@BC exhibit high adsorption capacity for heavy metals in strong acid environment. • –NH 2 and –COOH are favorable for the removal of heavy metals. • α-AC@BC has strong affinity with heavy metals to form steady coordination structure. [ABSTRACT FROM AUTHOR]

The application of plasma fuming technology opens up new horizons for the treatment of zinc-bearing residues. The present work uses a lab-scale Inductively Coupled Plasma (ICP) setup to investigate the hydrogen plasma reduction of ZnO and PbO from the CaO-FeO-SiO 2 based slags. Slag particles were melted when passing through the ICP torch, and the ZnO and PbO were reduced into Zn and Pb metal vapor by H 2 molecules and H radicals in the thermal hydrogen plasma. The metal vapor condensed on the particle surface when the particles passed through the plasma torch tail due to the high cooling rate. The PbO and ZnO content increased toward the particle core, implying the PbO and ZnO reduction from the slag particle surface. The increase in H 2 to Ar ratio or H 2 flow rate, power input and S content of the slags accelerated the process. [Display omitted] • The utilization of hydrogen plasma led to the reduction of ZnO and PbO, converting them into metal vapor. • The effects of process parameters on the reduction and volatilization of ZnO and PbO were identified. • A comprehensive understanding of the reduction mechanism and kinetics was achieved. • The hydrogen plasma process shows promise as a potential technology for the treatment of zinc-bearing residues. [ABSTRACT FROM AUTHOR]

Biomass-coal blending combustion is an effective method for utilizing biomass; however, its pollutant emission requires attention. Herein, the effect of biomass-coal blending combustion on lead (Pb) transformation was explored. Combustion experiments were conducted in a fixed-bed reactor, using coal, corn stalk, rice stalk, bamboo flour and their mixtures as fuels, at 1000, 1100, 1200 and 1300 °C. The Pb release ratios were determined by measuring its content in the fuels and solid-phase combustion products. The distribution of Pb forms was analyzed using sequential chemical extraction. The results indicate that blending combustion significantly enhanced the release of Pb. At blending ratio 1:1, the release ratios increased by 1.54–27.2%, 5.30–15.6%, and 2.31–7.76% at 1000, 1100, and 1200 °C, respectively. The potassium (K) components in biomass, mainly KCl and K 2 CO 3 , had a significant promoting effect on Pb release. K compounds facilitated the release of residual Pb through reactions with aluminosilicates. The promotion effect weakened as the temperature increased due to the faster evaporation rate of K. When the mass fractions of K in the fuels were equal, K 2 CO 3 exhibited a stronger promoting effect. HCl had minimal impact on the transformation of Pb. The results are helpful for optimizing the combination of biomass and coal to control Pb emission from the blending combustion source. [Display omitted] • The effect of biomass-coal blending combustion on lead (Pb) transformation was studied. • The release of Pb in coal was promoted during blending combustion. • K compounds in biomass promoted the release of Pb, while Cl had little effect. • K 2 CO 3 and KCl could both react with aluminosilicate and release residual Pb. • K 2 CO 3 had a stronger promoting effect on Pb release than KCl. [ABSTRACT FROM AUTHOR]

Mutations that activate oncogenes and deactivate tumor suppressor genes are widely recognized as significant contributors to cancer development. We investigated relationships between heavy metal exposure and the frequencies and types of gene mutations in patients with lung adenocarcinoma (LUAD) and colorectal cancer (CRC). Plasma concentrations of arsenic (As), cadmium (Cd), chromium (Cr), mercury (Hg), and lead (Pb) were measured using inductively coupled plasma mass spectrometry (ICP MS), and next-generation sequencing (NGS) of 1123 cancer-related genes was performed using the tumor tissues. Through Bayesian kernel machine regression (BKMR) analysis, we found associations between the integrated concentrations of the heavy metals and the number of gene mutations, especially insertions/deletions (indels), and Pb, As, and Cd were found to be the most significant contributors to the increased mutation rates. We extracted previously established mutational signatures and observed that they exhibit significant correlations with metal exposure. Moreover, we detected substantial shifts in the mutational landscape when comparing groups with high and low metal exposures. Several frequently mutated genes displayed positive correlations with metal exposure, whereas EGFR indels showed a negative association with Cd exposure. These findings suggest that heavy metal exposure can impact genomic stability in cancer-related genes, underscoring the importance of heavy metal exposure in cancer development. [Display omitted] • The integrated level of five heavy metals correlated with gene mutation rates. • Mutational signatures were positively or negatively linked to metal levels. • The variant frequencies of specific genes showed associations with metal levels. [ABSTRACT FROM AUTHOR]

Microalgal biomasses have been established as promising biosorbents for biosorption to remove heavy metal ions (HMIs) from wastewaters and contaminated natural waterbodies. Understanding the mechanism is important for the development of cost-effective processes for large scale applications. In this paper, a simple mathematical model was proposed for the predication of biosorption capacity of HMI by microalgal cells based on single cell mass, cell size, and HMI radius. One fundamental assumption based on which this model was developed, i.e., the biosorption of HMI by microalgal cells is predominantly monolayer bio-adsorption, was established based on kinetic, isothermal, FTIR, and Pb(II) distribution data generated in this study and in literature. The model was validated using a combination of experimental and literature data as well, demonstrating its capability to provide reasonable estimations although with discrepancies. The biosorption capacities of HMIs (mmol/g) by Chlorella vulgaris were experimentally determined to be in the following order: Pb(II)(0.360)> Zn(II)(0.325)> Cu(II)(0.254)> Ni(II)(0.249)> Cd(II)(0.235)> Co(II)(0.182). We systematically investigated the deviations of the predicted biosorption capacities in term of the effects of a few important parameters that were unaccounted for in the model, including the nanostructures on cell surface, HMI electronegativity, and biosorption buffer pH. Results suggest that the nanostructures on cell wall, likely the hairlike fibers, might be the primary locations where the binding sites for HMI were housed. Furthermore, isothermal data, which is suported by the predictions of this model, indicate the each effective binding site on C. vulgaris cell surface could bind to more than one Co(II) in biosorption while each of the other five HMIs tested in this study required more than one binding sites., Competing Interests: Declaration of Competing Interest The authors declare that they have no known competing financial interests or personal relationships that could have appeared to influence the work reported in this paper., (Copyright © 2023 Elsevier B.V. All rights reserved.)

Lead-zinc (Pb-Zn) tailings pose a significant environmental threat from heavy metals (HMs) contamination. Revegetation is considered as a green path for HM remediation. However, the interplay between HM transport processes and soil microbial community in Pb-Zn tailings (especially those in production) remain unclear. This study investigated the spatial distribution of HMs as well as the crucial roles of the soil microbial community (i.e., structure, richness, and diversity) during a three-year revegetation of production Pb-Zn tailings in northern Guangdong province, China. Prolonged tailings stockpiling exacerbated Pb contamination, elevating concentrations (from 10.11 to 11.53 g/kg) in long-term weathering. However, revegetation effectively alleviated Pb, reducing its concentrations of 9.81 g/kg. Through 16 S rRNA gene amplicon sequencing, the dominant genera shifted from Weissella (44%) to Thiobacillus (17%) and then to Pseudomonas (comprising 44% of the sequences) during the revegetation process. The structural equation model suggested that Pseudomonas, with its potential to transform bioavailable Pb into a more stable form, emerged as a potential Pb remediator. This study provides essential evidence of HMs contamination and microbial community dynamics during Pb-Zn tailings revegetation, contributing to the development of sustainable microbial technologies for tailings management., Competing Interests: Declaration of Competing Interest The authors declare that they have no known competing financial interests or personal relationships that could have appeared to influence the work reported in this paper., (Copyright © 2023 Elsevier B.V. All rights reserved.)

Food security is the basic requirement for human health and social stability. However, food pollution risk caused by economic development models, e.g., industrial transfer, is poorly understood. In this study, we developed and tested a point-area-domain cross-disciplinary approach to quantitatively estimate patterns and processes of soil and rice heavy metal (Pb, Hg and Cd) pollution risk caused by the fourth global industrial transfer process in CG Province of China. Our results indicate that the industrial transfer policy caused obvious cross-regional soil Pb, Hg and Cd pollution and industrial transfer. Industrial transfer process largely exacerbated rice Hg pollution risk in industrial transfer undertaking areas, mainly in the west (29.18%), periphery (28.38%) and south (25.88%) regions. The concentration increase in the west ranged from 0.0001 to 0.0775 mg/kg (mean±S.D. 0.00327 ± 0.00447, median 0.0026 mg/kg). The most important industries regarding rice Hg pollution in industrial transfer undertaking areas included special equipment (26.53%)>metal processing (11.93%)>communication equipment (11.25%)>chemical product (10.90%)> electrical machinery and equipment (9.59%). The pollution transfer was mainly attributed to environmental regulations, factor endowment and industrial structure. Our study highlights the importance for the integration of industrial transfer into food security and poverty reduction plans in the developing regions. ENVIRONMENTAL IMPLICATION: Food security is the basic requirement for human health and social stability. However, food pollution risk caused by economic development models, e.g., industrial transfer, is poorly understood. We developed and tested a point-area-domain cross-disciplinary approach to quantitatively estimate patterns and processes of soil and rice heavy metal pollution risk caused by the fourth global industrial transfer process. Our results suggest that industrial transfer process largely exacerbated soil and rice Hg pollution risk in the southeast coastal areas of China. Our study highlights the importance for the integration of industrial transfer into food security strategies in developing countries., Competing Interests: Declaration of Competing Interest The authors declare that they have no known competing financial interests or personal relationships that could have appeared to influence the work reported in this paper., (Copyright © 2023 Elsevier B.V. All rights reserved.)

• Plasma selenium level was significantly positively associated with eGFR in a dose-dependent manner. • High plasma selenium and low red blood cell cadmium or lead levels significantly interacted to decrease the OR for CKD. • High plasma selenium and low red blood cell lead levels significantly interacted to increase the eGFR. This study aimed to determine the interaction of red blood cell cadmium and lead, total urinary arsenic, and plasma selenium in chronic kidney disease (CKD). We recruited 220 CKD patients as well as 438 gender- and age-matched controls, and we defined CKD as <60 mL/min/1.73 m2 estimated glomerular filtration rate (eGFR) for three or more consecutive months. Plasma selenium and red blood cell cadmium and lead concentrations were measured by ICP-MS. Urinary arsenic species were determined via HPLC-HG-AAS and were summed to determine the total urinary arsenic concentration. Plasma selenium was positively correlated to eGFR, and subjects with high plasma selenium levels (>243.90 μg/L) had a significantly lower odds ratio (OR) and 95% confidence interval (CI) (0.23, 0.13–0.42) for CKD compared to those with low plasma selenium levels (≤ 196.70 μg/L). High plasma selenium and low red blood cell cadmium or lead concentrations interacted to decrease the OR and 95% CI for CKD (0.12, 0.06–0.26; 0.09, 0.04–0.19). High plasma selenium and low red blood cell lead levels also interacted to increase the eGFR (20.70, 15.56–26.01 mL/min/1.73 m2). This study is the first to suggest that selenium modifies the eGFR and OR in CKD induced by environmental toxicants. [ABSTRACT FROM AUTHOR]

• A facile approach for transforming microbes into spongy biosorbents was developed. • Properties of biosorbents were dependent on the structure and composition of microbes. • Modified fungi had higher productivity but less adsorption amount than those bacteria. • As-prepared biosorbents exhibited high adsorption capacity and selectivity for Pb(II). • Ion exchange, complexation and microprecipitation were vital in Pb(II) removal. Lead pollution in industrial-derived water has become an increasingly serious concern. The development of adsorbents with excellent efficiency, selectivity and separability using diverse microorganisms is ideal for treating lead pollution. In this study, gram-negative bacteria Pseudomonas putida I3, gram-positive bacteria Microbacterium sp. OLJ1 and mycelial fungus Talaromyces amestolkiae Pb served as raw materials to facilely synthesize sponge-like biosorbents via a one-step method at room temperature. SEM, EDS, FTIR, 13C NMR, XRD and XPS were used for investigating the morphology and surface properties of these three biosorbents. The obtained biosorbents possessed the same three-dimensional porous structure but different productivities and mechanical strengths due to the similar chemical compositions and different cell structures of their microorganisms. Pb(II) adsorption on X-PI3, X-OLJ1 and X-TPb was fast and pH dependent, with maximal adsorption capacities of 345.02, 237.02 and 199.02 mg/g, respectively. The biosorbents had a high selectivity for Pb(II), while Pb(II) remarkably suppressed the adsorption of co-existing heavy metal ions. The analyses indicated that Pb(II) removal was mainly achieved by ion exchange reactions, surface complexation with heteroatom-containing functional groups and microprecipitation. The treatment effects of synthetic and real wastewater revealed that the as-prepared biosorbents are promising for Pb(II) removal. [ABSTRACT FROM AUTHOR]

Highlights • Comprehensive procedure to quickly seek biofactors responsible for metal sorption. • Flagellin of Serratia Se1998 demonstrated a super binding capacity towards Pb. • A potential remediation tool for Pb contamination in aquatic environment. Abstract Metal contaminants present persistent and deleterious threats to environmental ecosystems and human health. Microorganisms can rapidly develop protective mechanisms against metal toxicity, such as metallothionein production. The identification of biological factors related to these protective mechanisms is essential for effective metal remediation. This study presents a robust pathway to rapidly locate and characterize a Pb-binding flagellin in Serratia Se1998, which can bind Pb at a 16:1 Pb: protein ratio. A column gel electrophoresis system hyphenated with inductively coupled plasma mass spectrometry (ICP MS) was constructed to efficiently separate and identify Pb-binding proteins from the whole bacterial proteome. PCR and transgenic assays were used to elucidate the exact sequences and biological function of Pb-binding proteins and heterogeneous expression of Pb-binding flagellin in E. coli could significantly enhance Pb removal from aqueous solution by approximately 45%. This method provides a benchmark procedure to rapidly identify biological factors responsible for metal biosorption. Identification of this unique Pb-binding flagellin highlights that microorganisms can survive high metal stresses due to various complex biological pathways for metal detoxification and remediation. [ABSTRACT FROM AUTHOR]