1. What makes A. guillouiae SFC 500-1A able to co-metabolize phenol and Cr(VI)? A proteomic approach
- Author
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Luca Bini, Claudia Landi, Laura Bianchi, Paola S. González, Ornella Mailén Ontañon, Elizabeth Agostini, Alfonso Carleo, and Assunta Gagliardi
- Subjects
Chromium ,ACINETOBACTER ,Proteomics ,0301 basic medicine ,Environmental Engineering ,Health, Toxicology and Mutagenesis ,Biotecnología del Medio Ambiente ,030106 microbiology ,Glyoxylate cycle ,Flavoprotein ,Biotecnología Medioambiental ,INGENIERÍAS Y TECNOLOGÍAS ,Acinetobacter guillouiae ,Acinetobacter, Chromium (VI), Phenol, Proteomics, Simultaneous bioremediation ,03 medical and health sciences ,chemistry.chemical_compound ,Bioremediation ,Bacterial Proteins ,Environmental Chemistry ,Phenol ,SIMULTANEOUS BIOREMEDIATION ,Chromium (VI) ,Waste Management and Disposal ,Simultaneous bioremediation ,Acinetobacter ,biology ,ATP synthase ,CHROMIUM (VI) ,biology.organism_classification ,Pollution ,Citric acid cycle ,Biodegradation, Environmental ,030104 developmental biology ,chemistry ,Biochemistry ,PHENOL ,biology.protein ,PROTEOMICS ,Environmental Pollutants ,Bacteria - Abstract
Acinetobacter guillouiae SFC 500-1A is an environmental bacterium able to efficiently co-remediate phenol and Cr(VI). To further understand the molecular mechanisms triggered in this strain during the bioremediation process, variations in the proteomic profile after treatment with phenol and phenol plus Cr(VI) were evaluated. The proteomic analysis revealed the induction of the β-ketoadipate pathway for phenol oxidation and the assimilation of degradation products through TCA cycle and glyoxylate shunt. Phenol exposure increased the abundance of proteins associated to energetic processes and ATP synthesis, but it also triggered cellular stress. The lipid bilayer was suggested as a target of phenol toxicity, and changing fatty acids composition seemed to be the bacterial response to protect the membrane integrity. The involvement of two flavoproteins in Cr(VI) reduction to Cr(III) was also proposed. The results suggested the important role of chaperones, antioxidant response and SOS-induced proteins in the ability of the strain to mitigate the damage generated by phenol and Cr(VI). This research contributes to elucidate the mechanisms involved in A. guillouiae SFC 500-1A tolerance and co-remediation of phenol and Cr(VI). Such information may result useful not only to improve its bioremediation efficiency but also to identify putative markers of resistance in environmental bacteria. Fil: Ontañon, Ornella Mailén. Universidad Nacional de Río Cuarto; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina Fil: Landi, Claudia. Università degli Studi di Siena; Italia Fil: Carleo, Alfonso. Hannover Medical School; Alemania. Università degli Studi di Siena; Italia Fil: Gagliardi, Assunta. University of Trento; Italia. Università degli Studi di Siena; Italia Fil: Bianchi, Laura. Università degli Studi di Siena; Italia Fil: González, Paola Solange. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. Universidad Nacional de Río Cuarto; Argentina Fil: Agostini, Elizabeth. Universidad Nacional de Río Cuarto; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina Fil: Bini, Luca. Università degli Studi di Siena; Italia
- Published
- 2018