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Background: The human TP53 gene, also known as p53, encodes for the tumor protein 53 (p53), regulates the cell cycle and hence functions as a tumor suppressor. This study was aimed to investigate some properties of the TP53 gene and its product, such as the homologous protein sequences in different species, the common transcription factor binding sites on their promoters, their phylogenetic relationship, conserved domains, and their expression profiles by in silico biology approach. Methods: We investigated the homology, conserved domain, promoter and expression profiles of the TP53 gene in various species by using bioinformatics approaches. Results: Our results revealed that, p53 molecules which investigated among all organisms are conserved. They have three conserved domains (p53_TAD, p53 DNA_binding, and p53 tetramerization motif), some of which have full and truncated sub-domains. Human p53 proteins is similar to those of Pan troglodytes, Macaca mulatta, Macaca fascicularis and Chlorocebus aethiops. In contrast, Monodelphis domestica protein is the most diverse of human p53. With the multiple alignment strategy, protein and domain sequences of Equus asinus, Muntiacus muntjak vaginalis and Monodelphis domestica are predicted to have a truncation. The comparative screening of the promoters demonstrated that TP53 genes do not seem to have any common conserved transcription factor binding sites. Conclusions: This study demonstrates that p53 molecules from various species are well conserved in the process of evolution. Comparative screening of the promoter sequences and its homologues which are found in the human p53 and NCBI database revealed that there was no common transcription factor binding sites. Phylogenetic trees constructed using the neighbor-joining method (NJ) revealed a close evolutionary relationship of p53 in various species. [ABSTRACT FROM AUTHOR]