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Objective: To study the effects of characteristic Li-medicine Alpinia officinarum Hance on improving insulin resistance (IR), and provide scientific evidence for the adjuvant treatment T2DM. Methods: CCK8 kit was used to detect the viability of HepG2 cells with 0-200 µg/ mL Alpinia officinarum Hance extract (AOE) and 0-100 µmol/L galangin, and determine the administration concentration. The IR model was established by inducing HepG2 cells with high glucose for 48 hours, and rosiglitazone (ROSI) was used as the positive control drug. Furthermore, we detected intracellular and extracellular glucose contents in IR-HepG2 cells after dosing AOE and galangin by flow cytometry and glucose detection kit, and evaluated the effect of regulating glucose metabolism and IR. Western blot was used to detect the effects of AOE and galangin on the expression of glucose transporter-4 (GLUT4) in IR-HepG2 cells. Results: CCK8 kit test result showed that 0-200 µg/mL AOE and 0-50 µmol/L galangin had no significant effect on HepG2 cell viability. The results of flow cytometry and glucose detection kit showed that the glucose uptake and consumption of the IR model group induced by high glucose was significantly lower than that of the blank group, and thus the IR model was successfully established. Both 50 µg/mL AOE and 10, 20 µmol/L galangin significantly increased the glucose uptake and consumption of IR-HepG2 cells (P<0.05); Western blot experiment showed that AOE and galangin significantly increased GLUT4 level of IR-HepG2 cells (P<0.01). Conclusion: Alpinia officinarum Hance could promote the uptake and consumption of glucose in IR-HepG2 cells by up-regulating the expression of GLUT4 protein, thereby improving IR and enhancing insulin sensitivity. [ABSTRACT FROM AUTHOR]

Objective: To explore the changes of cannabinoid receptor (CB1) and AMPA receptor subunit GluA1 in the medial prefrontal cortex (mPFC) of depression model rats induced by chronic unpredictable mild stress (CUMS). Methods: 30 rats were randomly divided into three groups. The three-week model group was given a three-week CUMS model, the four-week model group was given a four-week CUMS model, and the control group was given no treatment. The behavior of rats were detected in each group, and the expression of CB1 and GluA1 protein in synaptosomes in mPFC brain region was detected by Western blot. Results: Compared with the control group, the surcose preference decreased, the feeding latency increased in the novel inhibition feeding test, and the immobility time increased in the forced swimming test in the four-week model group. However, after three weeks of CUMS, there was no obvious change in the behavior of rats compared with the control group, suggesting that four-week model of CUMS was successful. CUMS can reduce the expression level of CB1 and GluA1 protein in synaptosomes of mPFC brain area in four-week model group (P<0.05), but there was no significant difference between three-week model group and control group. Conclusion: Fourweek CUMS was more likely to lead to depressive-like behavior in rats, which may be closely related to the expression of CB1 and GluA1 in mPFC. [ABSTRACT FROM AUTHOR]

Objective: To establish the quality standard of Danmu exract syrup. Methods: TLC method was used to qualitatively identify Nauclea officinalis in Danmu exract syrup. The content of strictosamide and chlorogenic acid in Danmu exract syrup was determined by high efficiency axillary chromatography. Results: The spots were clear and there was no negative interference. The linear relationship of strictosamide was good in the range of 100~300ug/ml (R2=0.9997). The average recovery was 99.1% and RSD was 1.16%(n=9). Chlorogenic acid had a good linear relationship in the range of 6.7~20.2ug/ml (R2=0.9991). The average recovery was 99.9% and RSD was 1.10% (n=9). Conclusion: The standard is safe and reasonable, the method is simple, accurate and reproducible, and it can be used for the quality control of Danmu exract syrup. [ABSTRACT FROM AUTHOR]

Objective: To explore the possible mechanism of Qingfei Paibu Decoction in the treatment of COVID-19 from the perspective of cytokine storm by network pharmacology. Methods: The TCMSP and SymMap databases were used to screen out the active components and targets of Qingfei Paibu Decoction; The GeneCards database was used to screen the predicted targets of COVID-19; Two targets were mapped; The STRING database and Cytoscape3.7.2 software were used to construct the network diagram of active drag-ingredient-target and screen out the core components and targets; The GO enrichment analysis and KEGG pathway enrichment analysis were carried out by OmicShare cloud platform and David respectively. Results: A total of 52 potential targets of Qingfei Paibu Decoction for the treatment of COVID-19 were obtained, among which 17 were core targets related to inflammation, mainly including cytokines such as IL, IFN, TNF and chemokines. And 26 core components were obtained, including quercetin, luteolin, kaempferol, naringenin, and baicalein; The GO enrichment results showed 224 biological processes, 15 molecular functions and 33 cell components related to inflammation; There were 5 inflammatory signaling pathways in KEGG enrichment results, including TNF signaling pathway, Nod-like receptor signaling pathway, Toll-like receptor signaling pathway, MAPK signaling pathway and cytokine receptor interaction. Conclusion: Qingfei Paibu Decoction can inhibit cytokine storms by acting on multiple targets and pathways with multiple components and thus treat COVID-19. [ABSTRACT FROM AUTHOR]