1. Apoptosis-related mRNA expression profiles of ovarian cancer cell lines following cisplatin treatment
- Author
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Sung Jong Lee, Bee Hak Hong, Joo-Hee Yoon, Kwan Yong Choi, Hyung Jin Cha, Chang Wook Park, and Eung-Sam Kim
- Subjects
Cisplatin ,Programmed cell death ,endocrine system diseases ,DNA repair ,Obstetrics and Gynecology ,General Medicine ,Cell sorting ,Biology ,Bioinformatics ,medicine.disease ,female genital diseases and pregnancy complications ,Oncology ,Apoptosis ,Cell culture ,medicine ,Cancer research ,Original Article ,Ovarian cancer ,Gene ,medicine.drug - Abstract
Objective The aim of this study was to identify apoptosis-related genes of ovarian cancer cell lines following cisplatin treatment. Methods We used IC(50) values and fluorescence-activated cell sorting analysis to compare cell death in 2 ovarian cancer cell lines, namely, SKOV-3 and OVCAR-3, upon treatment with cisplatin. Moreover, the change in transcriptional levels of apoptosis-associated genes was measured with a dendron-modified DNA microarray. Results The protein levels for the up-regulated genes in each cell line were validated to identify the molecules that may determine the cellular behavior of cisplatin resistance. Eight genes were over-expressed in the 2 cell lines. The cisplatin-induced up-regulation of DAD1 in transcriptional and protein levels contributed to the cisplatin resistance of OVCAR-3, and the up-regulation of FASTK and TNFRSF11A in SKOV-3 resulted in its higher sensitivity to cisplatin than that of OVCAR-3. Conclusion In the present study, we have identified a set of genes responsible for apoptosis following cisplatin treatment in ovarian cancer cell lines. These genes may give information about the understanding of cisplatin-induced apoptosis in ovarian cancer.
- Published
- 2010