1. Sequences responsible for efficient replication of simian immunodeficiency virus SIVMND in cells of the monocyte/macrophage lineage
- Author
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Riri Shibata, Sayuri Sakuragi, Jun-Ichi Sakuragi, Meiko Kawamura, Akio Adachi, and Hiroyuki Sakai
- Subjects
viruses ,T cell ,Molecular Sequence Data ,Biology ,V3 loop ,Virus Replication ,medicine.disease_cause ,Genes, env ,Monocytes ,Virus ,Cell Line ,Viral envelope ,Virology ,medicine ,Animals ,Humans ,Gene ,Base Sequence ,Macrophages ,Monocyte ,Simian immunodeficiency virus ,medicine.anatomical_structure ,Organ Specificity ,Cell culture ,RNA, Viral ,Simian Immunodeficiency Virus - Abstract
We determined the susceptibility of monocytic cell lines to infection with viral strains derived from two infectious clones of simian immunodeficiency virus isolated from a mandrill. One of the strains, which replicates poorly in T cell lines, was found to grow more rapidly than the other in these cells. The viral determinant for this property was genetically mapped within the env gene encoding a surface protein. Six amino acid substitutions identified appeared to be located outside of the domains corresponding to human immunodeficiency virus type 1 env functional domains such as the CD4-binding and V3 loop regions.
- Published
- 1992
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