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Ultraviolet radiation B cause photoaging of the skin by enhancing the high expression of matrix metalloproteinases (MMPs) to degrade collagen and inhibit the synthesis of procollagen. Trehalose (TRE), a widely occurring natural disaccharide, has been shown to play a cytoprotective role in many pathological processes. However, few studies have evaluated whether it has anti-photoaging potential. In this study, we investigated the anti-photoaging and mechanism of action of TRE using Human immortalized keratinocytes (HaCaT) cells. The results indicated that TRE pretreatment effectively scavenges reactive oxygen species (ROS) accumulation induced by UVB and increases the content of certain endogenous antioxidant factors in HaCaT cells. TRE suppressed UVB-induced MMP expression by blocking mitogen-activated protein kinases (MAPKs), activator protein 1 (AP-1) and NF-κB signaling. TRE treatment increased procollagen I synthesis via activating transforming growth factor-β (TGF-β)/Smad pathway and demonstrated that TRE may be a potential natural disaccharide for photoaging therapy in the future.

Seahorse is been used as a traditional Chinese medicine and invigorant for many years. Among, the seahorse is considered to have anti-fatigue effects. However, few researches have demonstrated such an effect. This study, the anti-fatigue effect of a novel peptide purified (SH200) from the seahorse (Hippocampus abdominalis) was investigated in C57BL/6 mice. The effect of SH200 on exercise performance was assessed using a Rotarod test and clinical biochemical parameters. In addition, the expression levels of muscular-associated proteins were examined by western blot and immunohistochemical analysis. The results revealed that SH200 prolonged the running time of mice, reduced the expression of creatine, CK, LDH, l -lactate, Citrate, and increased the production serum glucose and FFA, hepatic glycogen, ATP, and SDH. Addition, SH200 also improved mitochondrial function and protected muscle fibers via AMMPK/PGC-1α signal pathway. Thus, the SH200 is a potential candidate natural agent that could be used to reduce physical fatigue.