1. Genotype Differencies in Direct Plant Regeneration from Stem Explants of Populus tremula in Turkey
- Author
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Turan Tank, Nermin Gozukirmizi, Mehmet Çalıkoėlu, Kasim Bajrović, Z Ipekci, Melih Boydak, Tijen Oėraş, Hülya Balkan, Korhan Tunçtaner, Hulki Tanriyar, Mümtaz Tulukçu, Öznur Özden, and Tahsin Akalp
- Subjects
Forestry ,04 agricultural and veterinary sciences ,010501 environmental sciences ,Biology ,01 natural sciences ,Somaclonal variation ,chemistry.chemical_compound ,Tissue culture ,Murashige and Skoog medium ,Micropropagation ,chemistry ,Callus ,Botany ,Shoot ,040103 agronomy & agriculture ,0401 agriculture, forestry, and fisheries ,Zeatin ,0105 earth and related environmental sciences ,Explant culture - Abstract
Plant regeneration from 9 clones ofPopulus tremula representing diverse genotypes originated from Turkey, has been studied in different media, such as Murashige and Skoog’s Medium (MS), Aspen Culture Medium (ACM) and Woody Plant Medium (WPM) with appropriate supplements of growth regulators. Regeneration efficiency on WPM containing 1 mg/l zeatin using stem explantsin vitro, was higher than on MS and ACM. In this system, the time required for shoot regeneration was 2 weeks, which was shorter by two weeks than that of previous reports. A strong genotype dependence was observed among the tested nine clones. The regeneration system, we describe here, did not involve the callus phase and thus was less prone to somaclonal variation. When adventitious microshoots were cultured on WPM supplemented with 0.5 mg/l or 2 mg/l indole-3-butyric acid (IBA), root formation was obtained after 1 week of culture. Adaptation of regenerated plantletsin vivo was 100%. This system for micropropagation ofPopulus tremula is expected to be suitable for studies on transformation of genes involved in lignin biosynthesis via modification of lignin content.
- Published
- 1998