8 results on '"Miyashita, K."'
Search Results
2. Heat treatment of scallop adductor muscle using superheated steam
- Author
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Abe, T. and Miyashita, K.
- Subjects
Nitrogen -- Environmental aspects ,Scallops -- Physiological aspects ,Scallops -- Nutritional aspects ,Steaming (Cookery) -- Research ,Business ,Food/cooking/nutrition - Abstract
The use of heat treatment of scallop adductor muscles showed that superheated steaming at 150 degree Celsius was an optimum temperature for scallop adductor muscles to avoid extractive nitrogen loss.
- Published
- 2007
3. Isolation of peptides with angiotensin I-converting enzyme inhibitory effect derived from hydrolysate of upstream chum salmon muscle
- Author
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Ono, S., Hosokawa, M., Miyashita, K., and Takahashi, K.
- Subjects
Muscle proteins -- Research ,Peptides -- Research ,Food research ,Business ,Food/cooking/nutrition - Abstract
The antihypertensive effect of the thermolysin hydrolysate of muscle proteins from upstream chum salmon in SHR is investigated. The resulting hydrolysate showed high inhibitory activity against angiotensin I-converting enzyme in vitro.
- Published
- 2003
4. Oxidative stability of lipids rich in EPA and DHA extracted from fermented scallop ovary.
- Author
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Hamaoka N, Shimajiri J, Abe M, Hosokawa M, and Miyashita K
- Subjects
- Amino Acids analysis, Animals, Antioxidants analysis, Female, Fermentation, Food Handling methods, Oxidation-Reduction, Seafood analysis, Soy Foods analysis, Glycine max chemistry, Tocopherols analysis, Yeasts metabolism, Docosahexaenoic Acids analysis, Eicosapentaenoic Acid analysis, Lipid Metabolism, Ovary chemistry, Pectinidae
- Abstract
A novel seafood paste was developed by the fermentation of scallop ovary using rice malt (koji) and yeast culture. Chemical analysis of the product showed the formation of high level of free amino acids and organic acids during the fermentation. The product color and flavor resembled to Japanese traditional soybean miso. The contents of total lipids (TLs) extracted from the fermented products were ranged from 9.18% to 11.59% or 11.38% to 13.57%/dry sample weight. Although the TL was rich in oxidatively unstable polyunsaturated fatty acids (PUFAs) such as eicosapentaenoic acid (EPA) and docosahexaenoic acid (DHA), little decrease was found in these PUFAs during the fermentation, showing the high oxidative stability of the TL from the fermented scallop ovary. Moreover, the oxidative stability of the TL extracted from the fermented products increased with increasing the fermentation time. This would be mainly due to the formation of lipid soluble antioxidants such as tocopherols, which might be derived from yeast used for fermentation., (© 2013 Institute of Food Technologists®)
- Published
- 2013
- Full Text
- View/download PDF
5. Dietary combination of fish oil and taurine decreases fat accumulation and ameliorates blood glucose levels in type 2 diabetic/obese KK-A(y) mice.
- Author
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Mikami N, Hosokawa M, and Miyashita K
- Subjects
- Acyl-CoA Oxidase metabolism, Adipose Tissue, White metabolism, Adipose Tissue, White pathology, Animals, Diabetes Mellitus, Type 2 complications, Diabetes Mellitus, Type 2 metabolism, Diabetes Mellitus, Type 2 pathology, Fatty Acid Synthases metabolism, Fatty Acids, Omega-3 analysis, Fish Oils administration & dosage, Fish Oils analysis, Functional Food analysis, Glucose Transporter Type 4 metabolism, Hyperinsulinism prevention & control, Hyperlipidemias prevention & control, Leptin blood, Leptin metabolism, Liver enzymology, Male, Mice, Mice, Obese, Muscle, Skeletal enzymology, Obesity complications, Obesity metabolism, Obesity pathology, Seafood analysis, Taurine analysis, Adiposity, Diabetes Mellitus, Type 2 diet therapy, Fatty Acids, Omega-3 therapeutic use, Hyperglycemia prevention & control, Obesity diet therapy, Taurine therapeutic use
- Abstract
n-3 Polyunsaturated fatty acids (PUFAs) such as eicosapentaenoic acid (EPA) and docosahexaenoic acid (DHA), and taurine are functional compounds abundantly present in seafoods. In this study, we examined the combined effects of EPA- and DHA-rich fish oil and taurine on white adipose tissue (WAT) weight and blood glucose levels in diabetic/obese KK-A(y) mice. After a 4-wk administration of experimental diets (soybean oil or fish oil, supplemented with 0%, 2%, or 4% taurine), the increase in WAT weight of the mice fed the "fish oil + 4% taurine" diet was significantly suppressed compared to the "soybean oil + 4% taurine" and "fish oil only" diets. Serum triglycerides, free fatty acids, and total cholesterol levels decreased by fish oil administration. In addition, fish oil and taurine increased the activity of acyl-CoA oxidase, which is the rate-limiting enzyme of peroxisomal β-oxidation, increased in the liver of KK-A(y) mice. The activity of fatty acid synthase decreased by fish oil diets. Furthermore, blood glucose and insulin levels were significantly lower in the mice fed fish oil than in the soybean oil-fed mice. In fish oil + 4% taurine group, hyperglycemia and hyperinsulinemia were effectively improved in KK-A(y) mice compared to the fish oil only groups. In particular, the combination of fish oil and taurine enhanced the glucose transporter 4 (GLUT4) distribution in the plasma membrane of muscle tissue. These results suggest that EPA- and DHA-rich fish oil, especially in combination with taurine, exhibits preventive effects on WAT weight gain and hyperglycemia in diabetic/obese KK-A(y) mice., (© 2012 Institute of Food Technologists®)
- Published
- 2012
- Full Text
- View/download PDF
6. Comparative antioxidant activity of edible Japanese brown seaweeds.
- Author
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Airanthi MK, Hosokawa M, and Miyashita K
- Subjects
- Algorithms, Antioxidants isolation & purification, Complex Mixtures chemistry, Complex Mixtures isolation & purification, Food Preservatives analysis, Food Preservatives chemistry, Food Preservatives isolation & purification, Free Radical Scavengers analysis, Free Radical Scavengers chemistry, Free Radical Scavengers isolation & purification, Japan, Laminaria chemistry, Liposomes chemistry, Methanol chemistry, Oxidation-Reduction, Phenols analysis, Phenols chemistry, Phenols isolation & purification, Phosphatidylcholines chemistry, Sargassum chemistry, Solvents chemistry, Species Specificity, Time Factors, Xanthophylls analysis, Xanthophylls chemistry, Xanthophylls isolation & purification, Antioxidants analysis, Antioxidants chemistry, Seaweed chemistry
- Abstract
Japanese edible brown seaweeds, Eisenia bicyclis (Arame), Kjellmaniella crassifolia (Gagome), Alaria crassifolia (Chigaiso), Sargassum horneri (Akamoku), and Cystoseira hakodatensis (Uganomoku) were assayed for total phenolic content (TPC), fucoxanthin content, radical scavenging activities (DPPH, peroxyl radical, ABTS, and nitric oxide), and antioxidant activity in a liposome system. Among the solvents used for extraction, methanol was the most effective to extract total phenolics (TPC) from brown seaweeds. Among 5 kinds of brown seaweeds analyzed, methanol extract from C. hakodatensis was the best source for antioxidants. The high antioxidant activity of the extract was based not only on the high content of phenolics, but on the presence of fucoxanthin. No significant correlation (P > 0.05) was observed between TPC per gram extract with DPPH radical scavenging activity of the methanol extracts. These observed discrepancy would be due to structural variations in the phenolic compounds, and different levels of fucoxanthin in the extracts. The present study also demonstrated the synergy in the antioxidant activity of the combination of brown seaweed phenolics and fucoxanthin.
- Published
- 2011
- Full Text
- View/download PDF
7. Antiobesity effects of Undaria lipid capsules prepared with scallop phospholipids.
- Author
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Okada T, Mizuno Y, Sibayama S, Hosokawa M, and Miyashita K
- Subjects
- Adipose Tissue, White metabolism, Adiposity, Animals, Anti-Obesity Agents analysis, Anti-Obesity Agents chemistry, Fatty Acids, Omega-3 analysis, Food-Processing Industry economics, Gene Expression Regulation, Industrial Waste analysis, Industrial Waste economics, Ion Channels genetics, Ion Channels metabolism, Lipids blood, Lipids chemistry, Lipids therapeutic use, Male, Mice, Mice, Obese, Mitochondrial Proteins genetics, Mitochondrial Proteins metabolism, Nanocapsules therapeutic use, Obesity blood, Obesity metabolism, Phospholipids blood, Phospholipids chemistry, Phospholipids therapeutic use, RNA, Messenger metabolism, Random Allocation, Seaweed chemistry, Uncoupling Protein 1, Xanthophylls administration & dosage, Xanthophylls analysis, Anti-Obesity Agents therapeutic use, Dietary Supplements economics, Fatty Acids, Omega-3 therapeutic use, Obesity prevention & control, Pectinidae chemistry, Undaria chemistry, Xanthophylls therapeutic use
- Abstract
Based on previous research findings, a capsule was developed containing n-3 polyunsaturated fatty acid rich scallop phospholipids (PLs) with an incorporation of brown seaweed (Undaria pinnatifida) lipids (ULs) containing fucoxanthin. The antiobesity effects of the capsules were evaluated with an animal model using 3-wk-old male KK-A(y) mice. Each group received different combinations of lipid (UL, PL, UL + PL, or UL + PL capsule) either incorporated into the diet or into drinking water. Animals were sacrificed after a 4-wk experimental feeding period, and adipose tissues and organs were dissected and weighed. Blood samples were obtained to determine plasma lipid profiles. Uncoupling protein 1 (UCP1) mRNA expression levels were determined by real-time polymerase chain reaction analysis, and UCP1 expression was determined by western blotting analysis. Treatment with either UL alone or UL + PL (capsule) through drinking water resulted in a significant reduction in body weight, compared to the control group. The total white adipose tissue weight of mice fed the UL + PL capsule in drinking water was significantly reduced. Both UCP1 and UCP1 mRNA expression in epididymal fat from mice fed the capsule were significantly higher than in the control group. These results suggest that incorporation of UL into scallop-derived PL by means of capsulation may lead to an additive increase in the antiobesity properties of these bioactive lipids.
- Published
- 2011
- Full Text
- View/download PDF
8. Combination effect of herring roe lipids and proteins on plasma lipids and abdominal fat weight of mouse.
- Author
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Moriya H, Hosokawa M, and Miyashita K
- Subjects
- Abdominal Fat metabolism, Analysis of Variance, Animals, Body Composition, Body Weight, Dietary Fats, Unsaturated administration & dosage, Docosahexaenoic Acids administration & dosage, Docosahexaenoic Acids pharmacology, Eicosapentaenoic Acid administration & dosage, Eicosapentaenoic Acid pharmacology, Liver drug effects, Liver metabolism, Mice, Mice, Inbred C57BL, Mice, Inbred ICR, Organ Size, Palmitic Acid administration & dosage, Palmitic Acid pharmacology, Phospholipids administration & dosage, Phospholipids pharmacology, Random Allocation, Abdominal Fat drug effects, Cholesterol blood, Dietary Fats, Unsaturated pharmacology, Dietary Proteins pharmacology, Lipids blood
- Abstract
Dietary effects of herring roe lipids (HR-L) and proteins (HR-P) on plasma lipids and abdominal fat pad weight were determined. The main lipid class of HR-L was phospholipids (74%) and the main fatty acids were palmitic acid (16:0, 25.8%), DHA (22:6n-3, 21.6%), EPA (20:5n-3, 14.4%), and oleic acid (18:1n-9, 13.2%). A little increase in total cholesterol level was observed in plasma lipids of mouse fed with HR-L, although HR-L contained 9% cholesterol. This would be due to the lowering effect of EPA and DHA contained in HR-L on plasma cholesterol. Replacement of a part of dietary protein (5%) to HR-P reduced abdominal fat pad weight, but not significantly. On the other hand, combination of HR-P and HR-L significantly reduced the fat pad weight of the mouse as compared with the control. A significant effect of HR-P + HR-L was also observed in the reducing plasma lipid levels.
- Published
- 2007
- Full Text
- View/download PDF
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