1. Cell culture‐derived extracellular vesicles: Considerations for reporting cell culturing parameters
- Author
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Faezeh Shekari, Faisal J. Alibhai, Hossein Baharvand, Verena Börger, Stefania Bruno, Owen Davies, Bernd Giebel, Mario Gimona, Ghasem Hosseini Salekdeh, Lorena Martin‐Jaular, Suresh Mathivanan, Inge Nelissen, Esther Nolte‐’t Hoen, Lorraine O'Driscoll, Francesca Perut, Stefano Pluchino, Gabriella Pocsfalvi, Carlos Salomon, Carolina Soekmadji, Simon Staubach, Ana Claudia Torrecilhas, Ganesh Vilas Shelke, Tobias Tertel, Dandan Zhu, Clotilde Théry, Kenneth Witwer, and Rienk Nieuwland
- Subjects
cell culture‐conditioned medium ,cellular therapy ,ectosomes ,exosomes ,extracellular vesicles ,in vitro ,Cytology ,QH573-671 - Abstract
Abstract Cell culture‐conditioned medium (CCM) is a valuable source of extracellular vesicles (EVs) for basic scientific, therapeutic and diagnostic applications. Cell culturing parameters affect the biochemical composition, release and possibly the function of CCM‐derived EVs (CCM‐EV). The CCM‐EV task force of the Rigor and Standardization Subcommittee of the International Society for Extracellular Vesicles aims to identify relevant cell culturing parameters, describe their effects based on current knowledge, recommend reporting parameters and identify outstanding questions. While some recommendations are valid for all cell types, cell‐specific recommendations may need to be established for non‐mammalian sources, such as bacteria, yeast and plant cells. Current progress towards these goals is summarized in this perspective paper, along with a checklist to facilitate transparent reporting of cell culturing parameters to improve the reproducibility of CCM‐EV research.
- Published
- 2023
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