Search

Mitochondria-rich cells (MR cells) of the gills of rainbow trout undergo changes in relative distribution and biochemical function during acclimation to partialstrength (10‰) and full-strength (30‰) seawater. In isolated total gill cells, Na+/K+-ATPase activity increased fivefoid and H+-ATPase activity decreased fourfold when trout were acclimated to either 10&permi;; or 30‰ seawater. When total MR gill cells were separated based on differential binding to peanut lectin agglutinin (PNA), the PNA subtypes underwent a change in relative distribution in seawater-acclimated fish. In freshwater, the ratio of PNA-:PNA+ was 65:35 while in seawater the distribution changed to 20:80 PNA-:PNA+. Additionally, differential changes in Na+/K+-ATPase and H+-ATPase activity in each of the independent cell types occurred during seawater acclimation; Na+/K+-ATPase activity in the PNA- cells increased by 197% while in PNA+ cells Na+/K+ATPase decreased by 57%. However, H+-ATPase activity was decreased in both PNA- (84%) and PNA+ (72%) subtypes during acclimation to seawater. [ABSTRACT FROM AUTHOR]