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The article presents a study that compares single and touchdown polymerase chain reaction (PCR) procedures for detecting Pneumocystis jirovecii DNA in parrafin-embedded lung tissue samples. In this study, single and touchdown PCR were performed at two different gene loci, the mitochondrial large-subunit ribosomal RNA and the gene coding for dihydropteroate synthase. These two methods will determine which is the most effective for this comparison.

The article presents a study that evaluates polymerase chain reaction (PCR) methods for efficacy in detecting Pneumocystis jirovecii by analyzing oropharyngeal washings (OW) samples from individuals without Pneumocystis pneumonia (PcP) but might harbor the organism. Results indicate that analysis of PCR and OW, which can be obtained rapidly and by non-invasive procedures appears to be valuable for PcP diagnosis.

Examines the frequency at which healthy adult people in Seville are found sero-positive for harboring Pneumocystis organisms in Spain. Rate of spread of the infection; Variation of incidence in different geographical areas; Diagnosis of Pneumacystis carinii pneumonia.

Examines the lymphocyte response in patients with chronic pulmonary disease colonized with Pneumocystis jirovecii. Contribution of Pneumocystis carinii pneumonia (PcP) on the morbidity and mortality in the Western region; Development of PcP; Increase in the total number of lymphocytes and T cells.