1. Studies on Aldose Reductase Inhibitors from Fungi. I. Citrinin and Related Benzopyran Derivatives
- Author
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John M. Jacyno, Jack DeRuiter, R A Davis, and Cutler Hg
- Subjects
animal structures ,Stereochemistry ,Eye ,Biochemistry ,Structure-Activity Relationship ,chemistry.chemical_compound ,Aldehyde Reductase ,Animals ,Benzopyrans ,chemistry.chemical_classification ,Aldose reductase ,biology ,Fungi ,Penicillium ,Quinone methide ,Citrinin ,Rats ,Benzopyran ,Kinetics ,Enzyme ,chemistry ,Pyran ,Enzyme inhibitor ,biology.protein ,Molecular Medicine - Abstract
The fungal metabolites, citrinin (4,6-dihydro-8-hydroxy-3,4,5-trimethyl-6- oxo-3H-2-benzopyran-7-carboxylic acid) and DHMI (3,4-dihydro-6-methoxy-3,7-dimethyl-1H-2-benzopyran-8-ol), as well as certain synthetic derivatives, have been evaluated for aldose reductase inhibitory activity using a rat lens enzyme preparation. Citrinin and its reduction product, dihydrocitrinin, were found to have significant activity (IC50 approximately 10 microM), whereas the other compounds were 3-10 times less potent. Kinetic studies showed that citrinin was not an irreversible inhibitor of the enzyme, as might be expected of a quinone methide. Spectroscopic (NMR) evidence is presented for the existence of citrinin predominantly in the form of its hemi-acetal in aqueous solutions, suggesting that it is this benzo[c]pyran derivative which interacts with the enzyme, rather than the quinone methide form.
- Published
- 1992
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